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Metabolic Engineering and Adaptive Evolution of Clostridium beijerinckii To Increase Solvent Production from Corn Stover Hydrolysate.
Journal of Agricultural and Food Chemistry ( IF 6.1 ) Pub Date : 2020-07-02 , DOI: 10.1021/acs.jafc.0c03048
Jinle Liu 1 , Yu Jiang 2 , Jun Chen 3 , Junjie Yang 3 , Weihong Jiang 3 , Wei Zhuang 4, 5 , Hanjie Ying 4, 5 , Sheng Yang 2, 3
Affiliation  

The production of acetone–butanol–ethanol by solventogenic Clostridium using lignocellulosic biomass can be a potential alternative to petroleum-based butanol. However, previous studies on nondetoxified lignocellulose hydrolysate could not provide better results when compared to those in synthetic medium. In this study, we engineered the pentose pathway of Clostridium beijerinckii NCIMB 8052, which was then subjected to adaptive laboratory evolution in the gradient mixture of synthetic medium and pretreated corn stover enzymatic hydrolysate (CSH) prepared according to the National Renewable Energy Laboratory (NREL) standard. The final resultant strain CIBTS1274A produced 20.7 g/L of total solvents in NREL CSH diluted to 6% initial total sugars, supplemented with ammonium acetate. This performance was comparable with that of corn-based butanol. In addition, this strain was successfully used in the scale-up operation using nondetoxified corn stover and corncob hydrolysate at Lignicell Refining Biotechnologies Ltd., which once was the only commercial biobutanol industry in the world.

中文翻译:

拜氏梭菌的代谢工程和适应性进化,可提高玉米秸秆水解产物的溶剂产量。

使用木质纤维素生物质通过产溶剂梭菌生产丙酮-丁醇-乙醇可以替代石油基丁醇。但是,与合成培养基相比,以前对非排毒的木质纤维素水解产物的研究无法提供更好的结果。在这项研究中,我们设计了梭菌的戊糖途径NCIMB 8052,然后在合成培养基和根据国家可再生能源实验室(NREL)标准制备的预处理的玉米秸秆酶解产物(CSH)的梯度混合物中进行适应性实验室进化。最终得到的菌株CIBTS1274A在NREL CSH中产生20.7 g / L的总溶剂,稀释至初始总糖的6%,并补充乙酸铵。该性能与玉米基丁醇相当。此外,该菌株已在Lignicell Refining Biotechnologies Ltd.成功使用无毒玉米秸秆和玉米芯水解产物进行放大操作,该公司曾是世界上唯一的商业生物丁醇行业。
更新日期:2020-07-29
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