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Comparison of Nanomaterials for Delivery of Double-Stranded RNA in Caenorhabditis elegans.
Journal of Agricultural and Food Chemistry ( IF 6.1 ) Pub Date : 2020-07-01 , DOI: 10.1021/acs.jafc.0c02840
Stuart S Lichtenberg 1 , Jerome Laisney 1 , Zeinah Elhaj Baddar 2 , Olga V Tsyusko 1 , Subba R Palli 2 , Clement Levard 3 , Armand Masion 3 , Jason M Unrine 1
Affiliation  

RNA interference is a promising crop protection technology that has seen rapid development in the past several years. Here, we investigated polyamino acid biopolymers, inorganic nanomaterials, and hybrid organic–inorganic nanomaterials for delivery of dsRNA and efficacy of gene knockdown using the model nematode Caenorhabditis elegans. Using an oral route of delivery, we are able to approximate how nanomaterials will be delivered in the environment. Of the materials investigated, only Mg–Al layered double-hydroxide nanoparticles were effective at gene knockdown in C. elegans, reducing marker gene expression to 66.8% of that of the control at the lowest tested concentration. In addition, we identified previously unreported injuries to the mouthparts of C. elegans associated with the use of a common cell-penetrating peptide, poly-l-arginine. Our results will allow the pursuit of further research into promising materials for dsRNA delivery and also allow for the exclusion of those with little efficacy or deleterious effects.

中文翻译:

在秀丽隐杆线虫中递送双链RNA的纳米材料的比较。

RNA干扰是一种很有前途的作物保护技术,在过去几年中得到了迅速发展。在这里,我们使用线虫秀丽隐杆线虫模型研究了用于dsRNA传递的聚氨基酸生物聚合物,无机纳米材料和有机-无机杂化纳米材料的混合体。使用口服递送途径,我们能够估算出纳米材料在环境中的递送方式。在所研究的材料中,只有Mg-Al层状双氢氧化物纳米颗粒可有效改善秀丽隐杆线虫的基因敲低,在最低测试浓度下将标记基因的表达降低至对照组的66.8%。此外,我们发现了秀丽隐杆线虫口部先前未报告的损伤与使用常见的穿透细胞的肽聚-1-精氨酸有关。我们的结果将允许对有前景的用于dsRNA输送的材料进行进一步的研究,也可以排除疗效或有害作用很小的材料。
更新日期:2020-07-29
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