Reverse transcription recombinase polymerase amplification (RT-RPA), an isothermal nucleic acid amplification and detection method, was developed to detect peach latent mosaic viroid (PLMVd) in pollen and peach leaves. Results showed that this RT-RPA detection method can be performed at 42 °C and completed in approximately 5 min, and there was no cross-reactivity with other common peach viruses. A sensitivity assay showed that the RT-RPA assay was 1000-fold more sensitive than a regular RT-PCR. Moreover, the method was successfully applied to test field-collected samples. The newly developed RT-RPA assay is rapid, sensitive, and reliable method for detection of PLMVd in peach pollen and leaves and can be utilized as an effective technique in quarantine and viroid-free certification processes.

开发了一种等温核酸扩增和检测方法-逆转录重组酶聚合酶（RT-RPA），用于检测花粉和桃叶中的桃潜在花叶病毒（PLMVd）。结果表明，这种RT-RPA检测方法可以在42°C的温度下完成，并在大约5分钟内完成，并且与其他常见的桃子病毒没有交叉反应。灵敏度测定表明，RT-RPA测定的灵敏度比常规RT-PCR高1000倍。此外，该方法已成功应用于测试现场采集的样品。新开发的RT-RPA分析方法是检测桃花粉和叶片中PLMVd的快速，灵敏和可靠的方法，可作为一种有效的技术用于检疫和无病毒认证过程。