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Effectiveness of mesenchymal stem cell-seeded onto the 3D polylactic acid/polycaprolactone/hydroxyapatite scaffold on the radius bone defect in rat
Life Sciences ( IF 6.1 ) Pub Date : 2020-07-02 , DOI: 10.1016/j.lfs.2020.118038
A Oryan 1 , S Hassanajili 2 , S Sahvieh 1 , N Azarpira 3
Affiliation  

The importance of regeneration in large bone defects forces the orthopedic surgeons to search for a proper methodology. The present experiment evaluated the capability of polylactic acid/polycaprolactone/hydroxyapatite (PLA/PCL/HA) scaffold loaded with and without mesenchymal stem cells (MSCs) on bone regeneration. Fourier transform infrared spectrometry, X-ray diffraction, scanning electron microscopy, and rheology methodologies were used to characterize the scaffold. Forty Wistar rats were randomly divided into the four groups including the untreated defects as the control group and three other groups in which the bone defects were treated with autologous bones (autograft group), the PLA/PCL/HA scaffolds (PLA/PCL/HA group), and the MSCs-seeded scaffolds (MSCs-seeded PLA/PCL/HA group). Based on the qRT-PCR results, significantly higher expression levels of osteocalcin, osteopontin, and CD31 were seen in the cell-seeded scaffold group compared to the control group ( < ). The CT scanning and radiographic images depicted significantly more newly formed bonny tissue in the MSCs-loaded scaffold and autograft groups than the untreated group ( < ). The immunohistochemistry, biomechanical, histopathologic, and histomorphometric evaluations demonstrated significantly improved regeneration in the autograft and MSCs-loaded scaffold groups compared to the non-treated group ( < ). There were significant differences between the scaffold and untreated groups in all in vivo evaluations ( < ). The MSCs enhanced bone healing potential of the PLA/PCL/HA scaffold and the MSCs-seeded scaffold was comparable to the autograft as the golden treatment regimen ( > ).

中文翻译:

间充质干细胞接种到3D聚乳酸/聚己内酯/羟基磷灰石支架上对大鼠桡骨骨缺损的有效性

大骨缺损再生的重要性迫使骨科医生寻找合适的方法。本实验评估了负载和不负载间充质干细胞(MSC)的聚乳酸/聚己内酯/羟基磷灰石(PLA/PCL/HA)支架对骨再生的能力。使用傅里叶变换红外光谱、X射线衍射、扫描电子显微镜和流变学方法来表征支架。将40只Wistar大鼠随机分为4组,其中未处理骨缺损为对照组,另外3组采用自体骨(自体移植组)、PLA/PCL/HA支架(PLA/PCL/HA)处理骨缺损。组)和 MSC 接种支架(MSC 接种 PLA/PCL/HA 组)。根据 qRT-PCR 结果,与对照组相比,细胞接种支架组中骨钙素、骨桥蛋白和 CD31 的表达水平显着升高 (<)。 CT 扫描和放射线图像显示,加载 MSC 的支架和自体移植组中新形成的骨组织明显多于未治疗组 (<)。免疫组织化学、生物力学、组织病理学和组织形态学评估表明,与未治疗组相比,自体移植物和装载 MSC 的支架组的再生能力显着改善 (<)。在所有体内评估中,支架组和未处理组之间存在显着差异(<)。 MSCs 增强了 PLA/PCL/HA 支架的骨愈合潜力,并且 MSCs 接种支架与自体移植物相当,是黄金治疗方案 ( > )。
更新日期:2020-07-02
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