Abstract Identification of Fritillaria bulbus species was investigated by pyrolysis-gas chromatography combined with fingerprint analysis. An amount of 0.2 mg of sample powder was pyrolyzed in a vertical micro-furnace pyrolyzer at 470 °C, and the pyrolysis products were directly taken into a gas chromatography with a flame ionization detector or a mass spectrometer. A total of 15 samples from different Fritillaria bulbus species were characterized by the relative areas of 32 common peaks. The established method had a good reproducibility and the fingerprints showed a high similarity. In addition, the discrimination of Fritillaria bulbus samples from different species was performed by principal component analysis and hierarchical cluster analysis on the basis of the obtained database. The results proved the Py-GC fingerprint combined with statistical approach could be a good supplementary method for the traditional morphology-based taxonomy identification.

中文翻译：

---

贝母热解-气相色谱指纹图谱模式识别研究

摘要 采用热解-气相色谱结合指纹图谱对贝母种进行鉴定。取0.2mg样品粉末在立式微炉热解器中470℃热解，热解产物直接带火焰离子化检测器或质谱仪进入气相色谱仪。来自不同贝母种的总共 15 个样品通过 32 个常见峰的相对面积进行表征。建立的方法重现性好，指纹图谱相似度高。此外，在获得的数据库的基础上，通过主成分分析和层次聚类分析对不同物种的贝母样品进行了区分。