Athelia rolfsii TEMG (MH 236106) an exopolysaccharide (EPS) producing fungal strain was isolated and identified. Extraction, purification, characterization, antimicrobial, antioxidant, antiviral and antitumor activities of the polysaccharide were investigated. It characterized as a homopolysaccharide of glucose with a molecular weight of 345.622 kDa. The identification of the polysaccharide was conducted using scanning electron microscopy, energy dispersive X-ray analysis, ¹H and ¹³C NMR spectra. The existence of β-1,3 and β-1,6 linkages suggests that EPS could be scleroglucan. The purified scleroglucan showed considerable antibacterial and antioxidant activities. The results indicated that, there was no cytotoxicity on normal cell (W138) and no effect on tumor cell lines including HepG2 and PC3 showing IC50 of 5096.83, 5885.80 and 4803.90 μg/mL, respectively. The results showed also that Sclg could reduce the cytopathic effect by 50% (EC₅₀) at 15 and 50 μg/mL of herpes simplex virus type-1 (HSV-1) and influenza virus (H5N1), respectively.

罗耳阿太菌TEMG（MH 236106）的外多糖（EPS）生产的真菌菌株分离和鉴定。研究了多糖的提取，纯化，表征，抗菌，抗氧化，抗病毒和抗肿瘤活性。其表征为分子量为345.622kDa的葡萄糖的同多糖。使用扫描电子显微镜，能量色散X射线分析，¹ H和¹³进行多糖的鉴定1 H NMR谱。β-1,3和β-1,6键的存在表明EPS可能是硬葡聚糖。纯化的硬葡聚糖显示出相当大的抗菌和抗氧化活性。结果表明，对正常细胞（W138）没有细胞毒性，对包括HepG2和PC3在内的肿瘤细胞系也没有影响，IC50分别为5096.83、5888.50和4803.90μg/ mL。结果还表明，Sclg可以在15和50μg/ mL的单纯疱疹病毒1型（HSV-1）和流感病毒（H5N1）分别降低50％（EC ₅₀）的细胞病变作用。