OBJECTIVE To evaluate ovarian stimulation regimens and reproductive outcomes in a cohort of women undergoing ovarian tissue cryopreservation (OTC) and ovarian tissue transplantation (OTT). DESIGN Retrospective cohort study. SETTING University hospital and fertility clinics. PATIENTS Twenty-eight women undergoing OTT and in vitro fertilization (IVF) from 2012 to 2017. INTERVENTION OTC, OTT, and IVF. MAIN OUTCOME MEASURES Ovarian stimulation and IVF outcomes. RESULTS In total, 99 cycles were performed in 28 patients. In 19 patients responding to stimulation, a median of 3.0 cycles per patient (range: 1-14 cycles) was performed, and 2.0 mature oocytes were retrieved per cycle. The empty follicle rate was 35.9%. Eleven women achieved 15 pregnancies, of which 60% were lost during the first or second trimester, resulting in 5 of 28 women having ≥1 live births, and seven healthy children being born. In breast cancer patients (mean age at OTC: 33.0 years), the pregnancy rates (PR) and live birth rates (LBR) were 35.0% and 5.0% per embryo transfer, respectively. Patients aged ≥34.5 years at OTC all had breast cancer and did not achieve any pregnancies. For all other diagnoses (mean age at OTC: 26.6 years), PR and LBR were 50.0% and 37.5% per embryo transfer, respectively. Collectively, 39% of patients conceived at least once, and 17.9% delivered. Frozen-thawed embryo transfer (FET) resulted in more pregnancies than did fresh embryo transfer. CONCLUSION Our results suggest that women of advanced maternal age undergoing OTC and IVF have a poor ovarian reserve, resulting in a poor reproductive outcome. Interestingly, FET appeared to be superior to fresh transfer.

中文翻译：

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一组移植冷冻卵巢组织的丹麦女性进行体外受精治疗后的生殖结果

目的 评估一组接受卵巢组织冷冻保存 (OTC) 和卵巢组织移植 (OTT) 的女性的卵巢刺激方案和生殖结果。设计 回顾性队列研究。设置大学医院和生育诊所。患者 28 名女性在 2012 年至 2017 年期间接受了 OTT 和体外受精 (IVF)。干预 OTC、OTT 和 IVF。主要结果测量卵巢刺激和试管婴儿结果。结果 总共有 28 名患者进行了 99 个周期。在对刺激有反应的 19 名患者中，每名患者平均进行了 3.0 个周期（范围：1-14 个周期），每个周期回收了 2.0 个成熟卵母细胞。空卵泡率为 35.9%。11 名女性成功怀孕 15 次，其中 60% 在妊娠早期或中期怀孕，导致 28 名妇女中有 5 名活产≥1 次，并出生 7 名健康儿童。在乳腺癌患者中（OTC 平均年龄：33.0 岁），每次胚胎移植的妊娠率 (PR) 和活产率 (LBR) 分别为 35.0% 和 5.0%。OTC 年龄≥34.5 岁的患者均患有乳腺癌且未怀孕。对于所有其他诊断（OTC 的平均年龄：26.6 岁），每次胚胎移植的 PR 和 LBR 分别为 50.0% 和 37.5%。总的来说，39% 的患者至少怀孕了一次，17.9% 的患者分娩了。冻融胚胎移植 (FET) 比新鲜胚胎移植导致更多怀孕。结论 我们的结果表明，接受 OTC 和 IVF 的高龄产妇卵巢储备较差，导致生殖结果较差。有趣的是，