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"Turn-on" ratiometric electrochemical detection of H2O2 in one drop of whole blood sample via a novel microelectrode sensor.
Biosensors and Bioelectronics ( IF 12.6 ) Pub Date : 2020-07-02 , DOI: 10.1016/j.bios.2020.112402
Hui Dong 1 , Yanli Zhou 2 , Yuanqiang Hao 2 , Le Zhao 2 , Shuo Sun 2 , Yintang Zhang 2 , Baoxian Ye 3 , Maotian Xu 1
Affiliation  

Oxidative stress plays an important role in the pathogenesis of many diseases, while the exact mechanism that hydrogen peroxide (H2O2) as one of the most abundant reactive oxygen species (ROS) exerts its influence on oxidative stress remains unclear. We developed a novel turn-on ratiometric electrochemical sensor for the detection of H2O2 in blood samples. The electrochemical probe 5-(1,2-dithiolan-3-yl)-N-(4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl)pent-anamide (BA) was designed and synthesized for the selective detection of H2O2 via a one-step amide reaction. Meanwhile, Nile Blue A (NB) was optimized as an internal reference molecule, thus enabling accurate quantification of H2O2 in a complex environment. BA and NB were then co-assembled onto a carbon fiber microelectrode (CFME) coated with Au cones. The oxidation peak current ratio between BA and NB demonstrated good linearity with the logarithm of the H2O2 concentration values ranging from 0.5 μM to 400 μM with a low detection limit of 0.02 μM. The developed sensor showed remarkable selectivity against potential interferences in whole blood samples, especially for ascorbic acid, uric acid, and dopamine. In combination with the unique characteristics of CFME, such as a small size and good biocompatibility, the microsensor was used for rapid analysis of one drop of whole blood sample. This sensor not only creates a new platform for the detection of H2O2 in whole blood samples, but also provides a new design strategy of other ROS analysis for early diagnosis of ROS-related diseases, drug discovery processes, and pathological mechanisms.



中文翻译:

通过新型微电极传感器对一滴全血样本中的H2O2进行“开启”比例电化学检测。

氧化应激在许多疾病的发病机理中起着重要作用,而作为最丰富的活性氧(ROS)之一的过氧化氢(H 2 O 2)对氧化应激施加影响的确切机制仍不清楚。我们开发了一种新颖的比率式电化学传感器,用于检测血液样本中的H 2 O 2。电化学探针5-(1,2-二硫杂环戊-3-基)-N-(4-(4,4,5,5-四甲基-1,3,2-二氧杂硼烷-2-基)苯基)戊酰胺(BA)被设计和合成用于H 2 O 2的选择性检测通过一步酰胺反应。同时,优化了尼罗河蓝A(NB)作为内部参考分子,从而能够在复杂环境中准确定量H 2 O 2。然后将BA和NB共组装到涂有Au锥的碳纤维微电极(CFME)上。BA和NB之间的氧化峰电流比与H 2 O 2的对数表现出良好的线性关系浓度范围从0.5μM到400μM,检测下限为0.02μM。研发的传感器对全血样品,特别是抗坏血酸,尿酸和多巴胺的潜在干扰显示出显着的选择性。结合CFME的独特特性(例如小尺寸和良好的生物相容性),该微传感器用于快速分析一滴全血样品。该传感器不仅为检测全血样品中的H 2 O 2创造了一个新的平台,而且还提供了其他ROS分析的新设计策略,用于ROS相关疾病,药物发现过程和病理机制的早期诊断。

更新日期:2020-07-16
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