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Biodiscoloration, Detoxification and Biosorption of Reactive Blue 268 by Trametes sp. M3: a Strategy for the Treatment of Textile Effluents
Water, Air, & Soil Pollution ( IF 2.9 ) Pub Date : 2020-07-02 , DOI: 10.1007/s11270-020-04723-7
Caroline Aparecida Vaz de Araújo , Alex Graça Contato , Guilherme Mauro Aranha , Giselle Maria Maciel , Charles Windson Isidoro Haminiuk , Fabíola Dorneles Inácio , Jean Henrique da Silva Rodrigues , Rosane Marina Peralta , Cristina Giatti Marques de Souza

The concern with the environment and the depletion of natural resources has aroused the interest for the rational use and recycling of water. Therefore, this study evaluated the capacity of the Trametes sp. M3 isolate in the bioremediation of Reactive Blue (RB) 268 dye and its potential for use as an adsorbent in the treatment of textile effluents. In a solid medium containing RB 268, the discoloration rate was 1.00 and the growth rate was 1.4 cm/day. When evaluated in the in vivo biodiscoloration process, 100% of the dye lost its color after 120 h. The oxidative enzyme laccase was found in cultures containing the dye with high activity, indicating that it underwent induction. The chromatogram after cultivation of the fungus showed that there was a change in the structure of RB 268. The mycelium of the culture with the dye was analyzed by FTIR, pointing to an adsorption of RB 268 or its metabolites despite the absence of the color. In the biosorption, the best results were obtained when the mycelium was treated with HCl. The toxicity of the medium decreased after the cultivation of the fungus allowing the survival of the microcrustaceans in the acute toxicity bioassay.

中文翻译:

Trametes sp。对活性蓝268的生物脱色,解毒和生物吸附。M3:纺织废水处理策略

对环境和自然资源枯竭的关注引起了人们对合理利用和循环用水的兴趣。因此,这项研究评估了Trametes的能力sp。M3在活性蓝(RB)268染料的生物修复中分离出,并有潜力用作吸附剂,用于处理纺织品废水。在含有RB 268的固体培养基中,变色率为1.00,生长率为1.4cm /天。当在体内生物变色过程中进行评估时,120小时后100%的染料会失去颜色。在含有高活性染料的培养物中发现了氧化酶漆酶,表明它已被诱导。真菌培养后的色谱图表明RB 268的结构发生了变化。通过染料FTIR分析了带有染料的培养菌丝体,尽管没有颜色,但指出RB 268或其代谢产物被吸附。在生物吸附中,用HCl处理菌丝体可获得最佳结果。
更新日期:2020-07-02
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