Abstract

Lightweight, flexible electrodes based on three-dimensional (3D) graphene have attracted increasing attention owing to their potential applications in many fields. In this work, we developed 3D graphene through different methods and evaluated the potential of these materials as free-standing electrodes. Finally, the 3D graphene synthesized by hydrothermal gelation with cobalt ions as a cross-linking agent had the best electrochemical sensor performance. With our focus on practical applications, we further modified polydopamine and concanavalin A onto the 3D graphene to make it suitable for the detection of glucose. Under the optimum conditions, the impedance change in the free-standing electrode and the concentration of glucose have a good linear relationship in the range of 0.05–0.35 μmol/L and a limit of detection of 0.01 μmol/L. The electrochemical biosensor exhibited good sensitivity, selectivity, reproducibility, and stability. The fabricated biosensor was employed for the detection of glucose in mouse serum samples and provided satisfactory results. Therefore, the synthesized 3D graphene offers a new opportunity for immobilizing other biomolecules and fabricating biosensors.

Graphic abstract

Preparation of functionalized three-dimensional graphene free-standing electrochemical sensor.

中文翻译：

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三维石墨烯自立式电化学传感器的制备及其在葡萄糖测定中的潜在应用

摘要

由于其在许多领域中的潜在应用，基于三维（3D）石墨烯的轻型，柔性电极已引起越来越多的关注。在这项工作中，我们通过不同的方法开发了3D石墨烯，并评估了这些材料作为独立式电极的潜力。最后，以钴离子为交联剂通过水热凝胶合成的3D石墨烯具有最佳的电化学传感器性能。着眼于实际应用，我们将聚多巴胺和伴刀豆球蛋白A进一步修饰到3D石墨烯上，使其适合检测葡萄糖。在最佳条件下，自支撑电极的阻抗变化与葡萄糖浓度在0.05–0.35μmol/ L的范围内具有良好的线性关系，检出限为0.01μmol/ L。电化学生物传感器表现出良好的灵敏度，选择性，再现性和稳定性。所制备的生物传感器用于小鼠血清样品中葡萄糖的检测，并提供令人满意的结果。因此，合成的3D石墨烯为固定其他生物分子和制造生物传感器提供了新的机会。

图形摘要

功能化三维石墨烯自立式电化学传感器的制备。