Background

Many efforts have been performed in the last decade to accomplish the genomic and proteomic characterization of pediatric adamantinomatous craniopharyngioma with the purpose to elucidate the molecular mechanisms underlying the onset and development of this pediatric brain tumor, its high recurrence rate, and, although classified as a histologically benign neoplasm, its aggressive behavior.

Methods

The focus of this review is to perform the new comparison of the proteomic profiles of the solid component and the intracystic fluid of adamantinomatous craniopharyngioma based on our previous results, obtained by both the top-down and the bottom-up proteomic approaches, to disclose differences and similarities, and to discuss the results in the context of the most recent literature.

Results and conclusions

Proteins and peptides identified in the cyst fluid and in the solid component of adamantinomatous craniopharyngioma (AC) include beyond markers of inflammation (i.e., alpha-defensins), proteins involved in cell migration and protein degradation (i.e., beta-thymosin and ubiquitin peptides), whose main role might be in tumor growth and infiltration of the surrounding neural structures. These last appeared different in the solid components compared with the cyst fluid, missing their terminal part in the solid tissue, a feature generally associated to malignancies, which might represent a distinct molecular site for an aggressive behavior of AC.

中文翻译：

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精铜质颅咽管瘤：蛋白质组学研究的进展。

背景

在过去的十年中，已经进行了许多努力来完成小儿金刚起瘤性颅咽管瘤的基因组学和蛋白质组学研究，目的是阐明这种小儿脑肿瘤的发病和发展的分子机制，其高复发率，尽管分类为组织学上良性肿瘤，其侵袭性行为。

方法

本综述的重点是根据我们先前的结果（通过自上而下和自下而上的蛋白质组学方法获得的结果），对金刚鼻型颅咽管瘤的固体成分和囊内液的蛋白质组学特征进行新的比较，以揭示差异和相似性，并在最新文献的背景下讨论结果。

结果与结论

在囊肿液和金刚狼疮性咽喉瘤（AC）的固体成分中鉴定出的蛋白质和肽包括炎症标志物（即，α-防御素），参与细胞迁移和蛋白质降解的蛋白质（即，β-胸腺素和泛素肽） ，其主要作用可能是肿瘤生长和周围神经结构的浸润。与囊肿液相比，这些最后出现的固体成分有所不同，缺少了它们在实体组织中的末端部分，这是通常与恶性肿瘤相关的特征，这可能代表AC侵略性行为的独特分子部位。