Sporotrichosis, a mycosis caused by pathogenic species of the genus Sporothrix, affects diverse species of mammals. Until 2007, Sporothrix schenckii was considered the unique etiologic agent of sporotrichosis. Canine sporotrichosis is a poorly reported disease, and the majority of cases are from Rio de Janeiro, Brazil. There are scarce studies on the characterization of canine isolates of Sporothrix schenckii complex, as well as few antifungal susceptibility data available. The aim of this study was to characterize the clinical isolates of Sporothrix from dogs from Brazil at species level and evaluate their antifungal susceptibility profile. Polyphasic taxonomy was used to characterization at species level (morphological, phenotypical characteristics, and molecular identification). Antifungal susceptibility profiles (amphotericin B, itraconazole, ketoconazole, posaconazole, and terbinafine) were determined using the Clinical and Laboratory Standards Institute broth microdilution method (M38-A2). According to phenotypic identification and molecular analysis, 46 isolates included in this study were identified as S. brasiliensis and one as S. schenckii. Amphotericin B presented the highest minimum inhibitory concentration values, and the other drugs showed effective in vitro antifungal activity. This is the first report of S. schenckii in dogs from Brazil, since S. brasiliensis is the only species that has been described in canine isolates from Rio de Janeiro to date. Nevertheless, no differences were observed in the antifungal susceptibility profiles between the S. brasiliensis and S. schenckii isolates, and it is important to continuously study new canine clinical isolates from Rio de Janeiro, Brazil.

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犬孢子丝菌病：巴西流行地区孢子丝菌属的多相分类学和抗真菌药敏谱

孢子丝菌病是一种由孢子丝菌属病原菌引起的真菌病，影响多种哺乳动物。直到 2007 年，申克孢子丝菌被认为是孢子丝菌病的独特病原体。犬孢子丝菌病是一种鲜为人知的疾病，大多数病例来自巴西里约热内卢。关于申克孢子丝菌复合体的犬分离物特征的研究很少，可用的抗真菌药敏数据也很少。本研究的目的是在物种水平上表征来自巴西犬的孢子丝菌的临床分离物，并评估它们的抗真菌敏感性。多相分类法用于物种水平的表征（形态、表型特征和分子鉴定）。抗真菌药敏谱（两性霉素 B、伊曲康唑、酮康唑、泊沙康唑和特比萘芬）使用临床和实验室标准研究所肉汤微量稀释法 (M38-A2) 测定。根据表型鉴定和分子分析，本研究中46株被鉴定为巴西链球菌，1株为申克链球菌。两性霉素 B 的最低抑菌浓度值最高，其他药物显示出有效的体外抗真菌活性。这是巴西犬中申克链球菌的第一份报告，因为巴西链球菌是迄今为止在里约热内卢的犬分离物中被描述的唯一物种。然而，在 S. brasiliensis 和 S. schenckii 分离株之间的抗真菌敏感性谱中没有观察到差异，