In the coal biogasification, butyric acid is an important intermediate product. The enrichment of butyric acid-producing bacteria in coal geological methanogens is critical to confirm this assertion. Therefore, to study a method for enrichment of butyric acid-producing bacteria and to explore characteristic factors for evaluating the enrichment effect would be the basis for further strain isolation and metabolomics research. In this study, the nutrition control method was used for the butyric acid-producing bacteria enrichment from concentrated bacteria solution in Sihe coal seam. The characteristic factors’ changes in gas production, gas composition, butyric acid concentration, and pH were observed and analyzed in the experiment. High-throughput sequencing was used as a verification method to validate the medium and genera enrichment effect that can be used for the butyric acid-producing bacteria. Through experimental research and analysis, it was identified that the glucose-sucrose-maltose medium was the beneficial medium to the enrichment of butyric acid-producing bacteria, and the high-throughput sequencing determined that the enriched genera were Clostridium spp. Glucose-sucrose-maltose medium experimental data confirmed that the decrease of CO₂ and H₂ daily yield, the increase of butyric acid concentration, and the decrease of pH value had a significant positive correlation with the enrichment of Clostridium spp.

在煤的生物气化中，丁酸是重要的中间产物。煤地质产甲烷中富含丁酸的细菌的富集对于证实这一观点至关重要。因此，研究一种产丁酸细菌的富集方法，探索评价富集效果的特征因素，将成为进一步分离菌株和进行代谢组学研究的基础。本研究采用营养控制方法，从四合煤层浓缩菌液中富集丁酸菌。在实验中观察并分析了气体产生，气体组成，丁酸浓度和pH的特征因素变化。高通量测序被用作验证方法，以验证可用于产生丁酸细菌的培养基和属的富集效果。通过实验研究和分析，确定葡萄糖-蔗糖-麦芽糖培养基是富集丁酸生产细菌的有益培养基，高通量测序确定富集的属梭状芽孢杆菌属。葡萄糖-蔗糖-麦芽糖培养基的实验数据证实，CO ₂和H ₂日产量的下降，丁酸浓度的上升和pH值的下降与梭状芽孢杆菌的富集有显着的正相关。