A significant part of whey generated in Brazil is from small and mid-size dairy companies. Cheese whey has great potential for methane production through anaerobic biological processes but presents instabilities due to its high biodegradability. To study an alternative for the destination of this residue, the aim of this work was to investigate methane from the co-digestion of whey with glycerin and its environmental compliance at 55 °C in an anaerobic sequencing batch biofilm reactor. The best performance indicators were obtained with an influent composed of 88% cheese whey and 12% glycerin (by volume), fed-batch mode and organic loading rate of 19.3 kgCOD m⁻³ day⁻¹ at 55 °C. This operational condition allowed us to achieve a methane productivity of 203 molCH₄ m⁻³ day⁻¹, a methane yield close to theoretical value, and to remove 68% of all organic matter in the influent. A kinetic metabolic model was fitted to the experimental data, which indicated methanogenesis with preference for the acetoclastic route. Finally, the energy production (approximately 197 MWh month⁻¹) from an industrial scale reactor and its volume (three reactors of 152 m³) were assessed for a mid-size dairy industry, which could save up to US$ 22,000.00 per month in oil demand.

巴西生产的乳清粉很大一部分来自中小型乳制品公司。干酪乳清具有通过厌氧生物过程生产甲烷的巨大潜力，但由于其高生物降解性而存在不稳定的问题。为了研究这种残留物的替代方法，这项工作的目的是在厌氧测序批生物膜反应器中研究乳清与甘油共消化产生的甲烷及其在55°C下的环境顺应性。在55°C下，进水量为88％的奶酪乳清和12％的甘油（按体积计），分批补料模式和有机负载率为19.3 kgCOD m ^-3 天^-1时，可获得最佳性能指标。该操作条件使我们能够实现203 molCH ₄的甲烷生产率。 m ^-3 天^-1，甲烷收率接近理论值，可去除进水中68％的所有有机物。将动力学代谢模型拟合到实验数据，该模型表明甲烷生成优先于破碎屑途径。最后，评估了一个中型乳业的工业规模反应堆的能源生产（约197 MWh month ^-1）及其容量（三个反应堆152 m ³），这可以每月节省22,000.00美元。石油需求。