当前位置: X-MOL 学术Appl. Biol. Chem. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Costunolide inhibits inflammation in LPS-induced RAW264.7 cells and ameliorates gastric acid reflux-induced esophageal injury in rat model
Applied Biological Chemistry ( IF 3.2 ) Pub Date : 2020-07-01 , DOI: 10.1186/s13765-020-00514-0
Li Nan , Hyeon-Hwa Nam , Byung-Kil Choo

As one of the gastroesophageal reflux disease (GERD), reflux esophagitis (RE) severely affects patients’ daily lives. Costunolide (Cos), pertains to a sesquiterpene lactone, performs multiple pharmacological activities including inhibited acute liver injury, anti-inflammation and anti-oxidant. We carried out our study to investigate the anti-inflammatory effect and protective effects of Cos against esophageal tissue damage caused by gastric acid refluxing. The determination of anti-inflammatory effects of Cos were conducted using lipopolysaccharide (LPS)-induced RAW 264.7 cell inflammatory model. The ameliorative effects of Cos on RE were confirmed on RE controlled rats model. The results indicated that Cos reduced nitrite production and inhibited cellular inflammation via regulating the activation of NF-κB. In addition, gastric acid reflux increased expression levels of inflammatory proteins (COX-2, TNF-α and IL-1β) in esophageal tissues, while Cos treatment significantly downregulated the expression of these proteins by inhibiting activation of NF-κB. Furthermore, through observing histological stain, Cos significantly improved esophageal damage caused by gastric acid reflux. Therefore, we suggested that Cos has the potential to be a material of natural drug for the treatment of reflux esophagitis caused by acid reflux.

中文翻译:

Costunolide抑制LPS诱导的RAW264.7细胞发炎,并改善胃酸反流诱导的食管损伤

作为胃食管反流病(GERD)之一,反流性食管炎(RE)严重影响患者的日常生活。Costunolide(Cos)属于倍半萜内酯,具有多种药理活性,包括抑制急性肝损伤,抗炎和抗氧化剂。我们进行了研究,以研究Cos对由胃酸倒流引起的食管组织损伤的抗炎作用和保护作用。使用脂多糖(LPS)诱导的RAW 264.7细胞炎症模型确定Cos的抗炎作用。在稀土对照大鼠模型上证实了Cos对稀土的改善作用。结果表明,COS通过调节NF-κB的活化而减少了亚硝酸盐的产生并抑制了细胞炎症。此外,胃酸反流增加了食管组织中炎性蛋白(COX-2,TNF-α和IL-1β)的表达水平,而Cos处理通过抑制NF-κB的活化显着下调了这些蛋白的表达。此外,通过观察组织学染色,Cos显着改善了胃酸反流引起的食道损伤。因此,我们认为Cos有可能成为治疗由酸倒流引起的反流性食管炎的天然药物。
更新日期:2020-07-01
down
wechat
bug