Purpose. To observe the effect of low dose of lipopolysaccharide (LPS) pretreatment on the expression of CSF-1 and LRR-1 in rats with endotoxin-induced uveitis (EIU), and to explore the possible role of TLR4. Method. EIU was induced by a single subcutaneous injection of 200 μg LPS. For the endotoxin tolerance group, the induction of EIU was preceded by a daily subcutaneous injection of 0.1 mg/kg LPS for five days. Clinical scores were graded at 24 h after EIU under a slit lamp microscope. HE stain was performed to observe the histopathology. The concentrations of IL-17, INF-γ, and IL-6 in aqueous humor were quantified with enzyme-linked immunosorbent assay. Real-time PCR, Western blot, and immunofluorescence analysis were used to determine the expression of NF-κB P65 and the activation of CSF-1, LRR-1. Results: Low dose of LPS pretreatment produced a suppressive effect by significantly reducing the inflammatory reaction of anterior segment as measured by slit lamp and histopathology. It also significantly reduced the concentrations of IL-17, INF-γ, and IL-6 in aqueous humor and the expression of CSF-1 and NF-κB P65, while increased the expression of LRR-1 compared to the EIU group. Conclusions. Low dose of LPS pretreatment can ameliorate endotoxin-induced uveitis in rats. This protection may be associated with upregulation of LRR-1 and downregulation of CSF-1, which is regulated by TLR4 signaling pathway.

中文翻译：

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低剂量脂多糖预处理对大鼠内毒素性葡萄膜炎的保护作用与CSF-1下调和LRR-1上调有关。

目的。观察低剂量脂多糖（LPS）预处理对内毒素性葡萄膜炎（EIU）大鼠CSF-1和LRR-1表达的影响，探讨TLR4可能的作用。方法。通过单次皮下注射 200  μ g LPS 诱导 EIU。对于内毒素耐受组，在诱导 EIU 之前，每天皮下注射 0.1 mg/kg LPS，持续 5 天。在裂隙灯显微镜下，在 EIU 后 24 小时对临床评分进行分级。HE染色观察组织病理学。IL-17、INF- γ的浓度, 和房水中的 IL-6 用酶联免疫吸附测定法定量。实时荧光定量 PCR、蛋白质印迹和免疫荧光分析用于确定 NF- κ B P65 的表达和 CSF-1、LRR-1 的激活。结果：通过裂隙灯和组织病理学测量，低剂量的 LPS 预处理通过显着减少眼前节的炎症反应产生了抑制作用。与 EIU 组相比，它还显着降低了房水中 IL-17、INF- γ和 IL-6 的浓度以及 CSF-1 和 NF- κ B P65 的表达，同时增加了 LRR-1 的表达。结论. 低剂量LPS​​预处理可改善大鼠内毒素诱导的葡萄膜炎。这种保护可能与 LRR-1 的上调和 CSF-1 的下调有关，后者受 TLR4 信号通路的调节。