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Using liquid chromatography mass spectrometry (LC-MS) to assess the effect of age, high-fat diet, and rat strain on the liver metabolome.
PLOS ONE ( IF 3.7 ) Pub Date : 2020-07-01 , DOI: 10.1371/journal.pone.0235338
Greg Boyce 1 , Mohammad Shoeb 1 , Vamsi Kodali 1 , Terence Meighan 1 , Jenny R Roberts 1 , Aaron Erdely 1 , Michael Kashon 1 , James M Antonini 1
Affiliation  

The goal of this study was to use liquid chromatography mass spectrometry to assess metabolic changes of two different diets in three distinct rat strains. Sprague-Dawley, Fischer 344, and Brown-Norway male rats were maintained on a high-fat, or regular diet for 24 weeks. Liver tissue was collected at 4, 12, and 24 weeks to assess global small molecule metabolite changes using high resolution accurate mass spectrometry coupled to ultra-high-performance liquid chromatography. The results of the global metabolomics analysis revealed significant changes based on both age and diet within all three strains. Principal component analysis revealed that the influence of diet caused a greater variation in significantly changing metabolites than that of age for the Brown Norway and Fisher 344 strains, whereas diet had the greatest influence in the Sprague Dawley strain only at the 4-week time point. As expected, metabolites involved in lipid metabolism were changed in the animals maintained on a high fat diet compared to the regular diet. There were also significant changes observed in the concentration of Tri carboxylic acid cycle intermediates that were extracted from the liver of all three strains based on diet. The results of this study showed that a high fat diet caused significant liver and metabolic changes compared to a regular diet in multiple rat strains. The inbred Fisher 344 and Brown Norway rats were more metabolically sensitive to the diet changes than outbred Sprague Dawley strain. The study also showed that age, as was the case for Sprague Dawley, is an important variable to consider when assessing metabolic changes.



中文翻译:

使用液相色谱质谱法 (LC-MS) 评估年龄、高脂肪饮食和大鼠品系对肝脏代谢组的影响。

本研究的目的是利用液相色谱质谱法评估三种不同大鼠品系中两种不同饮食的代谢变化。Sprague-Dawley、Fischer 344 和 Brown-Norway 雄性大鼠维持高脂肪或常规饮食 24 周。在第 4、12 和 24 周时收集肝脏组织,使用高分辨率精确质谱法与超高效液相色谱法结合评估整体小分子代谢物变化。全球代谢组学分析的结果显示,所有三种菌株均因年龄和饮食而发生显着变化。主成分分析显示,对于 Brown挪威和 Fisher 344 菌株,饮食的影响导致代谢物显着变化的变化大于年龄,而饮食对 Sprague Dawley 菌株的影响最大,仅在 4 周时间点。正如预期的那样,与常规饮食相比,维持高脂肪饮食的动物中参与脂质代谢的代谢物发生了变化。根据饮食,从所有三种菌株的肝脏中提取的三羧酸循环中间体的浓度也观察到显着变化。这项研究的结果表明,与常规饮食相比,高脂肪饮食对多种大鼠品系造成了显着的肝脏和代谢变化。近交费舍尔 344 和布朗挪威大鼠比远交 Sprague Dawley 品系在代谢上对饮食变化更敏感。该研究还表明,年龄(就像斯普拉格·道利的情况一样)是评估代谢变化时需要考虑的一个重要变量。

更新日期:2020-07-01
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