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Sero-prevalence of arthropod-borne viral infections among Lukanga swamp residents in Zambia.
PLOS ONE ( IF 3.7 ) Pub Date : 2020-07-01 , DOI: 10.1371/journal.pone.0235322
Caroline C Chisenga 1 , Samuel Bosomprah 1, 2 , Kalo Musukuma 1 , Cynthia Mubanga 1 , Obvious N Chilyabanyama 1 , Rachel M Velu 1 , Young Chan Kim 3 , Arturo Reyes-Sandoval 3 , Roma Chilengi 1
Affiliation  

Introduction

The re-emergence of vector borne diseases affecting millions of people in recent years has drawn attention to arboviruses globally. Here, we report on the sero-prevalence of chikungunya virus (CHIKV), dengue virus (DENV), mayaro virus (MAYV) and zika virus (ZIKV) in a swamp community in Zambia.

Methods

We collected blood and saliva samples from residents of Lukanga swamps in 2016 during a mass-cholera vaccination campaign. Over 10,000 residents were vaccinated with two doses of Shanchol during this period. The biological samples were collected prior to vaccination (baseline) and at specified time points after vaccination. We tested a total of 214 baseline stored serum samples for IgG antibodies against NS1 of DENV and ZIKV and E2 of CHIKV and MAYV on ELISA. We defined sero-prevalence as the proportion of participants with optical density (OD) values above a defined cut-off value, determined using a finite mixture model.

Results

Of the 214 participants, 79 (36.9%; 95% CI 30.5–43.8) were sero-positive for Chikungunya; 23 (10.8%; 95% CI 6.9–15.7) for Zika, 36 (16.8%; 95% CI 12.1–22.5) for Dengue and 42 (19.6%; 95% CI 14.5–25.6) for Mayaro. Older participants were more likely to have Zika virus whilst those involved with fishing activities were at greater risk of contracting Chikungunya virus. Among all the antigens tested, we also found that Chikungunya saliva antibody titres correlated with baseline serum titres (Spearman’s correlation coefficient = 0.222; p = 0.03).

Conclusion

Arbovirus transmission is occurring in Zambia. This requires proper screening tools as well as surveillance data to accurately report on disease burden in Zambia.



中文翻译:

赞比亚卢坎加沼泽居民中节肢动物传播的病毒感染的血清流行率。

介绍

近年来,影响数百万人的媒介传播疾病的重新出现引起了全球虫媒病毒的关注。在这里,我们报告了赞比亚沼泽社区中的基孔肯雅热病毒(CHIKV),登革热病毒(DENV),马亚罗病毒(MAYV)和寨卡病毒(ZIKV)的血清流行情况。

方法

在2016年大规模霍乱疫苗接种运动中,我们从卢坎加沼泽居民那里采集了血液和唾液样本。在此期间,超过10,000名居民接种了两剂Shanchol 疫苗。在疫苗接种之前(基线)和疫苗接种后的指定时间点收集生物学样品。我们在ELISA上测试了总共214种基线存储的血清样品中的抗DENV和ZIKV的NS1和CHIKV和MAYV的E2的IgG抗体。我们将血清流行率定义为光密度(OD)值高于定义的临界值(使用有限混合模型确定)的参与者所占的比例。

结果

在214名参与者中,有79名(36.9%; 95%CI 30.5-43.8)的基孔肯雅血清阳性。寨卡(Zika)为23(10.8%; 95%CI 6.9-15.7),登革热为36(16.8%; 95%CI 12.1-22.5),玛雅罗为42(19.6%; 95%CI 14.5-25.6)。年龄较大的参与者更有可能感染寨卡病毒,而从事捕鱼活动的参与者则更容易感染基孔肯雅病毒。在所有测试的抗原中,我们还发现基孔肯雅人唾液抗体滴度与基线血清滴度相关(Spearman相关系数= 0.222; p = 0.03)。

结论

虫媒病毒正在赞比亚传播。这就需要适当的筛查工具以及监测数据,以准确报告赞比亚的疾病负担。

更新日期:2020-07-01
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