Cell division is a central and essential process in most bacteria, and also due to its complexity and highly coordinated nature, it has emerged as a promising new antibiotic target pathway in recent years. We have previously shown that ADEP antibiotics preferably induce the degradation of the major cell division protein FtsZ, thereby primarily leading to a depletion of the cytoplasmic FtsZ pool that is needed for treadmilling FtsZ rings. To further investigate the physiological consequences of ADEP treatment, we here studied the effect of ADEP on the different stages of FtsZ ring formation in rod-shaped bacteria. Our data reveal the disintegration of early FtsZ rings during ADEP treatment in Bacillus subtilis, indicating an essential role of the cytoplasmic FtsZ pool and thus FtsZ ring dynamics during initiation and maturation of the divisome. However, progressed FtsZ rings finalized cytokinesis once the septal peptidoglycan synthase PBP2b, a late stage cell division protein, colocalized at the division site, thus implying that the concentration of the cytoplasmic FtsZ pool and FtsZ ring dynamics are less critical during the late stages of divisome assembly and progression.

中文翻译：

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晚期卵母细胞的进展不受细胞质FtsZ库耗尽的影响

细胞分裂是大多数细菌的核心和必不可少的过程，并且由于其复杂性和高度协调的性质，近年来它已成为有希望的新的抗生素靶途径。先前我们已经表明，ADEP抗生素优选诱导主要的细胞分裂蛋白FtsZ降解，从而主要导致跑步FtsZ环所需的细胞质FtsZ库的消耗。为了进一步研究ADEP治疗的生理后果，我们在这里研究了ADEP对杆状细菌FtsZ环形成不同阶段的影响。我们的数据揭示了枯草芽孢杆菌在ADEP处理过程中早期FtsZ环的解体，表明胞质FtsZ池的重要作用，因此在分裂体的起始和成熟过程中FtsZ环的动力学也很重要。