当前位置: X-MOL 学术ACS Synth. Biol. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Bringing Light into Cell-Free Expression.
ACS Synthetic Biology ( IF 4.7 ) Pub Date : 2020-06-30 , DOI: 10.1021/acssynbio.0c00211
Peng Zhang 1 , Junzhu Yang 1 , Eunhee Cho 1 , Yuan Lu 1
Affiliation  

Cell-free systems, as part of the synthetic biology field, have become a critical platform in biological studies. However, there is a lack of research into developing a switch for a dynamical control of the transcriptional and translational process. The optogenetic tool has been widely proven as an ideal control switch for protein synthesis due to its nontoxicity and excellent time-space conversion. Hence, in this study, a blue light-regulated two-component system named YF1/FixJ was incorporated into an Escherichia coli-based cell-free system to control protein synthesis. The corresponding cell-free system successfully achieved a 5-fold dynamic protein expression by blue light repression and 3-fold dynamic expression by blue light activation. With the aim of expanding the applications of cell-free synthetic biology, the cell-free blue light-sensing system was used to perform imaging, light-controlled antibody synthesis, and light-triggered artificial cell assembly. This study can provide a guide for further research into the field of cell-free optical sensing. Moreover, it will also promote the development of cell-free synthetic biology and optogenetics through applying the cell-free optical sensing system to synthetic biology education, biopharmaceutical research, and artificial cell construction.

中文翻译:

将光带入无细胞表达。

作为合成生物学领域的一部分,无细胞系统已成为生物学研究的重要平台。但是,缺乏研究开发用于动态控制转录和翻译过程的开关。由于其无毒和出色的时空转换能力,光遗传学工具已被广泛证明是蛋白质合成的理想控制开关。因此,在这项研究中,将名为YF1 / FixJ的蓝光调节两组分系统并入了大肠埃希菌中。基于无细胞的系统来控制蛋白质合成。相应的无细胞系统通过蓝光抑制成功实现了5倍动态蛋白表达,并通过蓝光激活成功实现了3倍动态表达。为了扩展无细胞合成生物学的应用,无细胞蓝光传感系统用于成像,光控抗体合成和光触发人工细胞组装。这项研究可以为无细胞光学传感领域的进一步研究提供指导。此外,它将通过将无细胞光学传感系统应用于合成生物学教育,生物药物研究和人工细胞构建,促进无细胞合成生物学和光遗传学的发展。
更新日期:2020-08-21
down
wechat
bug