Ageing and parturition share common pathways, but their relationship remains poorly understood. Decidual cells undergo ageing as parturition approaches term, and these age-related changes may trigger labour. Mesenchymal stem/stromal cells (MSCs) are the predominant stem cell type in the decidua. Stem cell exhaustion is a hallmark of ageing, and thus ageing of decidual MSCs (DMSCs) may contribute to the functional changes in decidual tissue required for term spontaneous labour. Here, we determine whether DMSCs from patients undergoing spontaneous onset of labour (SOL-DMSCs) show evidence of ageing-related functional changes compared with those from patients not in labour (NIL-DMSCs), undergoing Caesarean section. Placentae were collected from term (37–40 weeks of gestation), SOL (n = 18) and NIL (n = 17) healthy patients. DMSCs were isolated from the decidua basalis that remained attached to the placenta after delivery. DMSCs displayed stem cell-like properties and were of maternal origin. Important cell properties and lipid profiles were assessed and compared between SOL- and NIL-DMSCs. SOL-DMSCs showed reduced proliferation and increased lipid peroxidation, migration, necrosis, mitochondrial apoptosis, IL-6 production and p38 MAPK levels compared with NIL-DMSCs (P < 0.05). SOL- and NIL-DMSCs also showed significant differences in lipid profiles in various phospholipids (phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol, phosphatidylserine), sphingolipids (ceramide, sphingomyelin), triglycerides and acyl carnitine (P < 0.05). Overall, SOL-DMSCs had altered lipid profiles compared with NIL-DMSCs. In conclusion, SOL-DMSCs showed evidence of ageing-related reduced functionality, accumulation of cellular damage and changes in lipid profiles compared with NIL-DMSCs. These changes may be associated with term spontaneous labour.

中文翻译：

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蜕膜间充质干/基质细胞的功能变化与自然分娩有关。

衰老和分娩有共同的途径，但它们之间的关系仍然知之甚少。随着分娩临近，蜕膜细胞会老化，这些与年龄相关的变化可能会引发分娩。间充质干/基质细胞 (MSC) 是蜕膜中的主要干细胞类型。干细胞耗竭是衰老的标志，因此蜕膜间充质干细胞 (DMSC) 的衰老可能导致足月自然分娩所需的蜕膜组织功能发生变化。在这里，我们确定来自接受自然分娩的患者 (SOL-DMSCs) 的 DMSCs 是否显示出与接受剖腹产的非分娩患者 (NIL-DMSCs) 的 DMSC 相比与衰老相关的功能变化的证据。从足月（妊娠 37-40 周）、SOL（n = 18）和 NIL（n = 17）健康患者收集胎盘。从分娩后仍附着在胎盘上的蜕膜基底中分离出 DMSC。DMSCs 表现出类似干细胞的特性并且是母体来源的。在 SOL-DMSC 和 NIL-DMSC 之间评估和比较了重要的细胞特性和脂质谱。与 NIL-DMSCs 相比，SOL-DMSCs 增殖减少，脂质过氧化、迁移、坏死、线粒体凋亡、IL-6 产生和 p38 MAPK 水平增加。P  < 0.05)。SOL-DMSC 和 NIL-DMSC 在各种磷脂（磷脂酰乙醇胺、磷脂酰甘油、磷脂酰肌醇、磷脂酰丝氨酸）、鞘脂（神经酰胺、鞘磷脂）、甘油三酯和酰基肉碱（P  <）中的脂质谱也显示出显着差异。总体而言，与 NIL-DMSCs 相比，SOL-DMSCs 的脂质谱发生了改变。总之，与 NIL-DMSCs 相比，SOL-DMSCs 显示出与衰老相关的功能降低、细胞损伤积累和脂质谱变化的证据。这些变化可能与足月自然分娩有关。