Abstract

Background

Rheumatoid arthritis (RA) is a joint destructive disorder. This study aimed to assess lncRNA MEG3 expression and its variant rs941576 in Egyptian patients with RA.

Subjects and methods

100 RA patients and 100 healthy individuals were enrolled in the study. Quantitative PCR was used for expression analysis and allelic discrimination technology for genotyping.

Results

LncRNA MEG3 was down-regulated in RA patients and negatively associated with RA clinical features and HIF-1α and VEGF serum levels. On the contrary, it was positively associated with BAX serum levels in RA patients. The major A allele of rs941576 variant was associated with RA patients (p = .0003). AA genotype showed a significant decrease in lncRNA MEG3 expression and BAX and increase in HIF-1α and VEGF.

Conclusions

Serum lncRNA MEG3 expression showed negative association with increased susceptibility to RA. MEG3 gene rs941576 (A/G) polymorphism was associated with increased severity of RA in the current population.

中文翻译：

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长链非编码 RNA MEG3 及其遗传变异 rs941576 与埃及患者的类风湿关节炎发病机制相关

摘要

背景

类风湿性关节炎 (RA) 是一种关节破坏性疾病。本研究旨在评估埃及 RA 患者中 lncRNA MEG3 的表达及其变体 rs941576。

主题和方法

研究招募了 100 名 RA 患者和 100 名健康个体。定量PCR用于基因分型的表达分析和等位基因鉴别技术。

结果

LncRNA MEG3 在 RA 患者中下调，并且与 RA 临床特征和 HIF-1α 和 VEGF 血清水平呈负相关。相反，它与RA患者的BAX血清水平呈正相关。rs941576 变异的主要 A 等位基因与 RA 患者相关 ( p  = .0003)。AA基因型显示lncRNA MEG3表达和BAX显着降低，HIF-1α和VEGF升高。

结论

血清 lncRNA MEG3 表达与 RA 易感性增加呈负相关。MEG3 基因 rs941576 (A/G) 多态性与当前人群中 RA 严重程度增加有关。