Abstract

This study aimed to evaluate the effect Cycloastragenol (CAG), a triterpenoid saponin isolated from the Radix astragali, on Aβ-induced BBB damage. An immortalized endothelial cell line (bEnd.3) was employed to mimic a BBB. The Western blot, TUNEL staining, Flow cytometric analysis and Enzyme-linked immunosorbent assay were performed. The present results showed that CAG (10, 50, 75 μM) can alleviate oligomer Aβ_1–42 induced bEnd.3 cell apoptosis and increase tight junction scaffold proteins expression. The result also indicated that CAG increased soluble Aβ efflux across BBB via upregulation of the P-gp and downregulation of RAGE expression.

摘要

本研究旨在评估从黄芪中分离出的三萜皂苷环黄芪素 (CAG) 对 Aβ 诱导的 BBB 损伤的影响。使用永生化的内皮细胞系 (bEnd.3) 来模拟 BBB。进行了蛋白质印迹、TUNEL 染色、流式细胞术分析和酶联免疫吸附测定。目前的结果表明，CAG (10, 50, 75 μM) 可以减轻寡聚体 Aβ _1-42诱导的 bEnd.3 细胞凋亡并增加紧密连接支架蛋白的表达。结果还表明，CAG 通过上调 P-gp 和下调 RAGE 表达增加了穿过 BBB 的可溶性 Aβ 流出。