Abstract

The reliable detection of nucleic acids at low concentrations in clinical samples like blood, urine and saliva, and in food can be achieved by nucleic acid amplification methods. Several portable and hand-held devices have been developed to translate these laboratory-based methods to point-of-care (POC) settings. POC diagnostic devices could potentially play an important role in environmental monitoring, health, and food safety. Use of a smartphone for nucleic acid testing has shown promising progress in endpoint as well as real-time analysis of various disease conditions. The emergence of smartphone-based POC devices together with paper-based sensors, microfluidic chips and digital droplet assays are used currently in many situations to provide quantitative detection of nucleic acid targets. State-of-the-art portable devices are commercially available and rapidly emerging smartphone-based POC devices that allow the performance of laboratory-quality colorimetric, fluorescent and electrochemical detection are described in this review. We present a comprehensive review of smartphone-based POC sensing applications, specifically on microbial diagnostics, assess their performance and propose recommendations for the future.

摘要

可以通过核酸扩增方法实现对血液、尿液和唾液等临床样本以及食品中低浓度核酸的可靠检测。已经开发了几种便携式和手持设备来将这些基于实验室的方法转化为即时 (POC) 设置。POC 诊断设备可能在环境监测、健康和食品安全方面发挥重要作用。使用智能手机进行核酸检测已在终点和各种疾病状况的实时分析方面取得了可喜的进展。基于智能手机的 POC 设备以及基于纸张的传感器、微流控芯片和数字液滴分析的出现目前在许多情况下用于提供核酸目标的定量检测。本综述描述了最先进的便携式设备是可商用的，并且快速出现的基于智能手机的 POC 设备可以实现实验室质量的比色、荧光和电化学检测。我们全面回顾了基于智能手机的 POC 传感应用，特别是微生物诊断，评估其性能并为未来提出建议。