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A Mouse Model for Infection with Enterovirus A71 in Small Extracellular Vesicles.
mSphere ( IF 4.8 ) Pub Date : 2020-07-01 , DOI: 10.1128/msphere.00377-20
Jiaqi Gu 1, 2 , Jing Wu 1, 2 , Yuwen Cao 1, 2 , Xinran Zou 1, 2 , Xiaonan Jia 1, 2 , Yiqian Yin 1, 2 , Li Shen 3 , Daihua Fang 4 , Lingxiang Mao 5
Affiliation  

Enterovirus A71 (EV-A71) is the major pathogen of hand, foot, and mouth disease (HFMD); in some severe cases, it could develop into central nervous system (CNS) disease such as aseptic meningitis, encephalitis, and neurogenic pulmonary edema in children under 5 years. The EV-A71 pathogenesis which is involved with the CNS is unclear due to the lack of a simple and reliable mouse model thus far. Most clinical EV-A71 isolates could not effectively infect the neonatal mouse, which used to be an EV-A71 infection model. The small extracellular vesicles (sEVs) released from clinical EV-A71 isolate-infected cells were infectious in cell lines and could cause a high viral replication in mice. Neonatal ICR mice were injected intraperitoneally with these infectious sEVs and showed more weight loss and higher mortality than those mice injected with the clinical EV-A71 isolate. By using these sEVs, we provided a simple and effective method by which we can generate a stable and valuable animal model for the studies of EV-A71 pathogenesis and therapy.

中文翻译:

在小细胞外囊泡中感染肠道病毒 A71 的小鼠模型。

肠道病毒 A71 (EV-A71) 是手足口病 (HFMD) 的主要病原体;严重者可发展为5岁以下儿童无菌性脑膜炎、脑炎、神经源性肺水肿等中枢神经系统疾病。由于迄今为止缺乏简单可靠的小鼠模型,与 CNS 相关的 EV-A71 发病机制尚不清楚。大多数临床 EV-A71 分离株不能有效感染新生小鼠,其曾经是 EV-A71 感染模型。从临床 EV-A71 分离感染细胞释放的小细胞外囊泡 (sEV) 在细胞系中具有传染性,并可能导致小鼠的高病毒复制。与注射临床 EV-A71 分离株的小鼠相比,新生 ICR 小鼠腹膜内注射这些感染性 sEV,显示出更多的体重减轻和更高的死亡率。通过使用这些 sEV,我们提供了一种简单有效的方法,通过该方法我们可以为 EV-A71 发病机制和治疗研究生成稳定且有价值的动物模型。
更新日期:2020-07-01
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