Polymorphisms of the myostatin gene (MSTN) have been studied in vertebrates including several aquaculture species, revealing their role in growth. We attempted to identify polymorphisms in MSTN associated with growth traits of juvenile farmed red sea bream Pagrus major, an important cultured fish species in Japan. Polymorphisms in the coding region of MSTN were screened, and six polymorphisms were found among three exons: a deletion in exon 1, a single nucleotide polymorphism (SNP) in exon 2 and four SNPs in exon 3. The deletion in exon 1 eliminated a codon (glutamine) from the region comprising the polyglutamine structure, but all SNPs were synonymous. We analysed the polymorphisms for association with growth phenotype in large and small P. major specimens obtained from commercial production at 50 days post hatching. Two SNPs located in exon 3 (c.+846T>C and c.+1140T>C) were significantly more frequent in the large group, while no SNPs were significantly associated with the small phenotype. Haplotypes were reconstructed using genotype information of the two phenotype groups, and nine haplotypes (Hap_1 to Hap_9) were successfully reconstructed. Hap_6 and Hap_7 were significantly more often observed in small and large groups respectively. Analysis of diplotypes (haplotype combinations) of individual specimens revealed 19 diplotypes. The Hap_7/Hap_7 diplotype was found in 46.7% of large phenotype specimens, significantly more frequently than in the small group (13.5%). These results will be useful for marker‐assisted selection of red sea bream to improve production with respect to growth.

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与幼年红鲷g草生长相关的肌生长抑制素基因的多态性和单倍型

已在包括几种水产养殖物种的脊椎动物中研究了肌肉生长抑制素基因（MSTN）的多态性，揭示了它们在生长中的作用。我们试图确定与日本人工养殖的​​重要红鱼鲷鱼幼体的生长特性相关的MSTN多态性。筛选了MSTN编码区的多态性，在三个外显子中发现了六个多态性：外显子1缺失，外显子2的单核苷酸多态性（SNP）和外显子3的四个SNP。外显子1的缺失消除了一个密码子。 （谷氨酰胺）来自包含多谷氨酰胺结构的区域，但所有SNP都是同义词。我们分析了在孵化后50天从商业生产获得的大型和小型体育标本中与生长表型相关的多态性。位于第3外显子的两个SNP（c。在大组中，+ 846T> C和c。+ 1140T> C）的频率明显更高，而没有SNP与小表型显着相关。利用两个表型组的基因型信息重建单倍型，并成功构建了九个单倍型（Hap_1至Hap_9）。Hap_6和Hap_7分别在小群体和大群体中更常见。单个样本的双倍型（单倍型组合）分析显示有19个双倍型。Hap_7 / Hap_7双倍型在大型表型标本中占46.7％，显着高于小型表型（13.5％）。这些结果将有助于红鲷鱼的标记辅助选择，以提高产量。而没有SNPs与小表型显着相关。利用两个表型组的基因型信息重建单倍型，并成功构建了九个单倍型（Hap_1至Hap_9）。Hap_6和Hap_7分别在小群体和大群体中更常见。单个样本的双倍型（单倍型组合）分析显示有19个双倍型。Hap_7 / Hap_7双倍型在大型表型标本中占46.7％，显着高于小型表型（13.5％）。这些结果将有助于红鲷鱼的标记辅助选择，以提高产量。而没有SNPs与小表型显着相关。利用两个表型组的基因型信息重建单倍型，并成功构建了九个单倍型（Hap_1至Hap_9）。Hap_6和Hap_7分别在小群体和大群体中更常见。单个样本的双倍型（单倍型组合）分析显示有19个双倍型。Hap_7 / Hap_7双倍型在大型表型标本中占46.7％，显着高于小型表型（13.5％）。这些结果将有助于红鲷鱼的标记辅助选择，以提高产量。成功地重建了九个单倍型（Hap_1至Hap_9）。Hap_6和Hap_7分别在小群体和大群体中更常见。单个样本的双倍型（单倍型组合）分析显示有19个双倍型。Hap_7 / Hap_7双倍型在大型表型标本中占46.7％，显着高于小型表型（13.5％）。这些结果将有助于红鲷鱼的标记辅助选择，以提高产量。成功地重建了九个单倍型（Hap_1至Hap_9）。Hap_6和Hap_7分别在小群体和大群体中更为常见。单个样本的双倍型（单倍型组合）分析显示有19个双倍型。Hap_7 / Hap_7双倍型在大型表型标本中占46.7％，显着高于小型表型（13.5％）。这些结果将有助于红鲷鱼的标记辅助选择，以提高产量。显着高于小组（13.5％）。这些结果将有助于红鲷鱼的标记辅助选择，从而提高产量。显着高于小组（13.5％）。这些结果将有助于红鲷鱼的标记辅助选择，以提高产量。