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Antihypertensive effect of quinoa protein under simulated gastrointestinal digestion and peptide characterization
Journal of the Science of Food and Agriculture ( IF 4.1 ) Pub Date : 2020-07-29 , DOI: 10.1002/jsfa.10609
Huimin Guo 1, 2 , Yuqiong Hao 1 , Aurore Richel 2 , Nadia Everaert 2 , Yinhuan Chen 1 , Mengjie Liu 1 , Xiushi Yang 1 , Guixing Ren 1
Affiliation  

BACKGROUND Quinoa protein is a potential source of bioactive peptides. Although some studies have demonstrated its angiotensin converting enzyme (ACE) inhibitory properties, investigation on their in vivo effect of blood pressure regulation and peptide characterization remain limited. RESULTS Quinoa proteins hydrolyzate (QPH) was prepared by simulated gastrointestinal digestion. In the short-term administration, QPH produced lowest SBP and DBP in SHRs at 6 h post oral administration, which effectively controlled blood pressure in SHRs. In vitro study showed that hydrolyzate of quinoa protein from simulated gastrointestinal digestion is capable of inhibiting ACE activity, which was attributed to the activity of a number of low-molecular-weight peptides. With relatively high scores predicted by PeptideRanker, three promising bioactive peptides, FHPFPR, NWFPLPR and NIFRPF, were further studied and their ACE inhibition effects were confirmed with IC50 values of 34.92, 16.77 and 32.40 μM, respectively. Molecular docking study provided insights into the binding of ACE with peptides, and revealed that the presence of specific amino acids in the peptide sequence (Pro, Phe and Arg at the C-terminal, and Asn at the N-terminal) could contribute to the interaction between ACE and peptides. CONCLUSION These results demonstrated the potential of QPH for the management of hypertension, which could be a good candidate of functional foods to control the high blood pressure. This article is protected by copyright. All rights reserved.

中文翻译:

模拟胃肠消化和肽表征下藜麦蛋白的抗高血压作用

背景藜麦蛋白是生物活性肽的潜在来源。尽管一些研究已经证明了其血管紧张素转化酶 (ACE) 抑制特性,但对其血压调节和肽表征的体内作用的研究仍然有限。结果藜麦蛋白水解物(QPH)是通过模拟胃肠道消化制备的。在短期给药中,QPH在口服后6小时SHRs产生最低的SBP和DBP,有效控制了SHRs的血压。体外研究表明,来自模拟胃肠道消化的藜麦蛋白水解物能够抑制 ACE 活性,这归因于许多低分子量肽的活性。根据 PeptideRanker 预测的相对较高的分数,三种有前途的生物活性肽,进一步研究了 FHPFPR、NWFPLPR 和 NIFRPF,它们的 ACE 抑制作用得到证实,IC50 值分别为 34.92、16.77 和 32.40 μM。分子对接研究为 ACE 与肽的结合提供了见解,并揭示了肽序列中特定氨基酸的存在(C 端的 Pro、Phe 和 Arg,以及 N 端的 Asn)可能有助于ACE 和肽之间的相互作用。结论 这些结果证明了 QPH 治疗高血压的潜力,它可能是控制高血压的功能性食品的良好候选者。本文受版权保护。版权所有。分子对接研究为 ACE 与肽的结合提供了见解,并揭示了肽序列中特定氨基酸的存在(C 端的 Pro、Phe 和 Arg,以及 N 端的 Asn)可能有助于ACE 和肽之间的相互作用。结论 这些结果证明了 QPH 治疗高血压的潜力,它可能是控制高血压的功能性食品的良好候选者。本文受版权保护。版权所有。分子对接研究为 ACE 与肽的结合提供了见解,并揭示了肽序列中特定氨基酸的存在(C 端的 Pro、Phe 和 Arg,以及 N 端的 Asn)可能有助于ACE 和肽之间的相互作用。结论 这些结果证明了 QPH 治疗高血压的潜力,它可能是控制高血压的功能性食品的良好候选者。本文受版权保护。版权所有。结论 这些结果证明了 QPH 治疗高血压的潜力,它可能是控制高血压的功能性食品的良好候选者。本文受版权保护。版权所有。结论 这些结果证明了 QPH 治疗高血压的潜力,它可能是控制高血压的功能性食品的良好候选者。本文受版权保护。版权所有。
更新日期:2020-07-29
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