Rahnella aquatilis is an important pathogen of several aquatic organisms and is found widely distributed in the freshwater, soil, fish and human clinical samples. Our previously published study reported a novel pathogenic R. aquatilis strain KCL‐5 to crucian carp (Carassius auratus ). To further investigate the characteristics and pathogenesis caused by R. aquatilis , we here report on the pathological changes, bacterial genomic and proteomic analyses of strain KCL‐5. Significantly pathological changes in liver, intestine, spleen and gills were observed in infected fish. The genome consists of one circular chromosome 5,062,299 bp with 52.02% GC content and two plasmids (506,827 bp, 52.16%; 173,433 bp, 50.00%) and predicted 5,653 genes, 77 tRNAs and 22 rRNAs. Some virulence factors were characterized, including outer membrane protein, haemolysin, RTX toxin, chemotaxis and T3SS secretion system. Antimicrobial resistance genes such as EmrAB‐TolC, MexABC‐OpmB and RosAB efflux pump were found in strain KCL‐5. KEGG analysis showed that mainly functional modules were ABC transporters, biosynthesis of amino acids, two‐component system, quorum sensing, flagellum assembly and chemotaxis, in which most of them were identified by using 2‐DE/MS analyses. To our knowledge, this was first report on the molecular characteristics of R. aquatilis by multi‐omics approaches, which will provide insights into the pathogenic mechanism of R. aquatilis infection in fish.

中文翻译：

---

关于report鱼致病性水生小球藻KCL-5的特征的首次报道：通过基因组和蛋白质组学分析揭示。

水生拉氏菌是几种水生生物的重要病原体，广泛分布于淡水，土壤，鱼类和人类临床样品中。我们先前发表的研究报告了to鱼（Carassius auratus）的一种新的致病性水产罗非鱼菌株KCL-5 。进一步调查水生罗非鱼的特征和致病机理，我们在这里报告了KCL-5菌株的病理变化，细菌基因组和蛋白质组学分析。在受感染的鱼中观察到肝，肠，脾和g的明显病理变化。该基因组由一个具有52.02％GC含量的5,062,299 bp环形染色体和两个质粒（506,827 bp，52.16％; 173,433 bp，50.00％）和预测的5,653个基因，77个tRNA和22个rRNA组成。表征了一些毒力因子，包括外膜蛋白，溶血素，RTX毒素，趋化性和T3SS分泌系统。在菌株KCL-5中发现了抗药性基因，例如EmrAB-TolC，MexABC-OpmB和RosAB外排泵。KEGG分析表明，主要功能模块是ABC转运蛋白，氨基酸的生物合成，两组分系统，群体感应，鞭毛组装和趋化性，其中大多数是通过2-DE / MS分析确定的。据我们所知，这是关于R. aquatilis由多组学方法，这将提供深入的致病机制R. aquatilis鱼类感染。