MicroRNAs(miRNAs) have been reported to regulate gene expression in many processes. MiRNA in extracellular vesicles (EVs) also have been widely investigated, while there is no studies of miRNAs in seminal EVs. Subgroup J of Avian leukosis virus (ALV-J) can be transmitted vertically, but the mechanism of it is not clear enough. This study was to examine the miRNA expression profile in seminal EVs and inquire into the relation between it and the vertical transmission by performing gene ontology (GO) and pathway enrichment analysis. Here, we first isolated and characterized seminal EVs by Nanoparticle Tracking Analysis、Western Blot and Transmission electron microscopy experiments. By deep sequencing of each EVs miRNA library, 9 typical differentially expressed miRNA, including 6 up-regulated and 3 down-regulated, were identified. Gene target prediction, GO annotation and KEGG pathway enrichment analysis showed possible function associated with these miRNAs. Overall, these findings will increase our understanding of the content and composition of miRNA in seminal EVs and provide new insights into the important role of the seminal EVs miRNAs regulation in ALV-J transmission.

MicroRNA（miRNA）已报道在许多过程中调节基因表达。还已经广泛研究了细胞外囊泡（EVs）中的miRNA，但尚未对精液EV中的miRNA进行研究。禽白血病病毒（ALV-J）的J亚组可以垂直传播，但其机制还不清楚。这项研究旨在检查精子电动汽车中的miRNA表达谱，并通过执行基因本体论（GO）和途径富集分析来探究其与垂直传播之间的关系。在这里，我们首先通过纳米粒子跟踪分析，Western印迹和透射电子显微镜实验分离并鉴定了精液电动汽车。通过每个EVs miRNA文库的深度测序，鉴定出9种典型的差异表达miRNA，包括6种上调和3种下调。基因靶点预测，GO注释和KEGG途径富集分析显示了与这些miRNA相关的可能功能。总体而言，这些发现将增进我们对精液电动车中miRNA含量和组成的了解，并为精液电动车miRNA调控在ALV-J传播中的重要作用提供新的见解。