Chaya is a neglected, fast-growing and drought-resistant shrub, mostly cultivated in Mesoamerica as vegetable and medicinal plant. The increasing demand for safe products promotes processes with non-toxic solvents. In this context, Subcritical Water Extraction (SWE) and Microwave-Assisted Extraction (MAE) with ethanol were firstly applied to recover extracts rich in phenolic compounds from Chaya leaves. Temperature influence of SWE and MAE was evaluated and compared to Soxhlet extraction (SOX). Results were expressed as extraction yield, total phenolic content (TPC), antioxidant capacity by DPPH and FRAP methods, and composition by LC–MS/MS. Global yield and TPC values from SWE and MAE increased with extraction temperature. MAE provided extracts with higher antioxidant activity compared to SWE and SOX. Kaempferol, quercetin and myricetin were the most abundant phenolic compounds found in Chaya the extracts. Besides, 21 of the phenolics evaluated were detected for the first time in Chaya leaves.

Chaya是一种被忽视，生长迅速且抗干旱的灌木，大多在中美洲作为蔬菜和药用植物种植。对安全产品日益增长的需求促进了使用无毒溶剂的工艺。在这种情况下，首先应用亚临界水萃取（SWE）和微波辅助乙醇萃取（MAE）从茶树叶片中回收富含酚类化合物的萃取物。评估了SWE和MAE的温度影响，并将其与索氏提取（SOX）进行了比较。结果表示为提取率，总酚含量（TPC），DPPH和FRAP方法的抗氧化能力以及LC-MS / MS的组成。SWE和MAE的总产量和TPC值随提取温度的升高而增加。MAE提供的提取物比SWE和SOX具有更高的抗氧化活性。山emp酚 槲皮素和杨梅素是茶叶提取物中最丰富的酚类化合物。此外，首次在Chaya叶中检测到21种所评估的酚类。