A surface-enhanced Raman scattering (SERS)-based lateral flow assay (LFA) strip using silica-encapsulated gold nanoparticles (Si-AuNPs) was developed for the diagnosis of mosquito-borne diseases—dengue fever, Zika virus, or malaria—which are endemic to tropical regions. To investigate the thermal stability of the SERS nanoprobes, Raman peak intensity variations for AuNPs and Si-AuNPs were measured and compared at 45°C. SERS mapping images of target human immunoglobulin (IgG) on the test line of the Si-AuNP-loaded LFA strip were also evaluated at the same temperature to test the reproducibility of detection. In this case, the distribution of Raman mapping points on the test line was relatively uniform compared with the AuNP-loaded LFA strips at 45°C. This indicates that the silica encapsulation on the surface of the AuNPs improves the reproducibility of the SERS nanoprobes at high temperatures. The limit of detection (LOD) value of the AuNP-loaded LFA strips was significantly increased when the temperature was increased from 25°C to 45°C. In the case of the Si-AuNP-loaded LFA strips, however, the LOD value at 45°C was almost the same as that at 25°C. This result also shows that the silica encapsulation of the AuNPs improves the thermal stability of the LFA strips at high temperatures. Thus, Si-AuNPs are reproducible and thermally stable labeling agents for use in LFA strips at high temperatures and are suitable for the preparation of rapid kits, which can be used in tropical areas.

使用二氧化硅包裹的金纳米颗粒（Si-AuNPs）开发了基于表面增强拉曼散射（SERS）的侧向流动测定（LFA）试纸，用于诊断蚊媒疾病-登革热，寨卡病毒或疟疾-是热带地区特有的。为了研究SERS纳米探针的热稳定性，在45°C下测量并比较了AuNPs和Si-AuNPs的拉曼峰强度变化。还在相同温度下评估了载有Si-AuNP的LFA条测试线上的目标人免疫球蛋白（IgG）的SERS映射图像，以测试检测的可重复性。在这种情况下，与在45°C下加载AuNP的LFA条带相比，测试线上的拉曼映射点分布相对均匀。这表明AuNPs表面的二氧化硅封装提高了SERS纳米探针在高温下的重现性。当温度从25°C升高到45°C时，AuNP负载的AuFA样品的检测限（LOD）值显着增加。但是，对于装有Si-AuNP的LFA胶条，在45°C下的LOD值几乎与25°C下的LOD值相同。该结果还表明，AuNP的二氧化硅包封改善了LFA条在高温下的热稳定性。因此，Si-AuNPs是可再现且热稳定的标记剂，可在高温下用于LFA试纸条中，并且适合制备可在热带地区使用的快速试剂盒。当温度从25°C升高到45°C时，AuNP负载的AuFA样品的检测限（LOD）值显着增加。但是，对于装有Si-AuNP的LFA胶条，在45°C下的LOD值几乎与25°C下的LOD值相同。该结果还表明，AuNP的二氧化硅包封改善了LFA条在高温下的热稳定性。因此，Si-AuNPs是可再现且热稳定的标记剂，可在高温下用于LFA试纸条中，并适合制备可在热带地区使用的快速试剂盒。当温度从25°C升高到45°C时，AuNP负载的AuFA样品的检测限（LOD）值显着增加。但是，对于装有Si-AuNP的LFA胶条，在45°C下的LOD值几乎与25°C下的LOD值相同。该结果还表明，AuNP的二氧化硅包封改善了LFA条在高温下的热稳定性。因此，Si-AuNPs是可再现且热稳定的标记剂，可在高温下用于LFA试纸条中，并且适合制备可在热带地区使用的快速试剂盒。该结果还表明，AuNP的二氧化硅包封改善了LFA条在高温下的热稳定性。因此，Si-AuNPs是可再现且热稳定的标记剂，可在高温下用于LFA试纸条中，并且适合制备可在热带地区使用的快速试剂盒。该结果还表明，AuNP的二氧化硅包封改善了LFA条在高温下的热稳定性。因此，Si-AuNPs是可再现且热稳定的标记剂，可在高温下用于LFA试纸条中，并且适合制备可在热带地区使用的快速试剂盒。