The effects of microplastics (MPs) on organisms have drawn a worldwide attention in the recent years. In this study, zebrafish embryos were employed to assess the combined effects of MPs and cadmium (Cd) on the aquatic organisms. Lethal and sublethal effects were recorded at 8, 24, 32, 48 and 96 hpe (hour post exposure, hpe). The exposure under a series concentration of MPs and/or an environmental level Cd has the negative impacts on survival and heart rate (HR). And there was a positive correlation between MPs concentration and lethal and sublethal toxicity under combined exposure. The physiological parameters showed that the mixture of two stressors had the antagonistic toxicity under low concentration of MPs (0.05, 0.1 mg/L) while the synergistic sublethal toxicity under high levels of MPs (1, 5, 10 mg/L) on zebrafish embryos. Both the scanning electron micrographs (SEM) and fluorescence microscope photos suggested an electrostatic interaction and weak physical forces generated between MPs and chorion membrane. It is inferred that the 10 μm MPs could induce the protective effect of chorion membrane and cause complex toxicities with Cd. But when it involved with other pollutants, the toxic effects and mechanism are still waiting to be figured out.

近年来，微塑料（MPs）对生物的影响已引起全世界的关注。在这项研究中，斑马鱼胚胎被用来评估MPs和镉（Cd）对水生生物的综合影响。在8、24、32、48和96 hpe（暴露后小时，hpe）下记录致死和亚致死作用。一系列MP和/或Cd环境浓度下的暴露会对存活率和心率（HR）产生负面影响。复合暴露下MPs浓度与致死和亚致死毒性呈正相关。生理参数表明，两种应激源混合物在低浓度MPs（0.05，0.1 mg / L）下对斑马鱼胚胎具有拮抗毒性，而在高浓度MPs（1、5、10 mg / L）下具有协同亚致死毒性。 。扫描电子显微照片（SEM）和荧光显微镜照片均表明MP与绒毛膜之间存在静电相互作用和弱的物理力。推测10μmMPs可能诱导绒毛膜的保护作用，并与Cd产生复杂的毒性。但是，当它与其他污染物结合时，其毒性作用和机理仍待弄清。