Abstract This paper presents a study on scale-up and cost reduction of the production of spores of Metarhizium anisopliae IBCB 425, entomopathogenic fungus used in sugarcane crops. Rice was mixed with sugarcane bagasse (9:1 w/w) for substrate composition, assuring adequate physical structure for cultivation in packed-beds. Spores yield from only rice in bench-scale packed-bed bioreactor was 56 % of the one obtained from the mixture 9:1 w/w. In comparison to plastic packages used in bioindustries, equivalent spores yields per gram of substrate have been achieved in bench and pilot-scale bioreactors built by cylindrical jacketed modules, that provide better control of operational and environmental variables, attested by little variability among replicates. Although non-negligible temperature rise (5 °C above the ideal) occurred within the pilot-scale bioreactor, spores production was not harmed in comparison to bench-scale. By reusing rice up to three successive cultivations, a 2.5-fold increase of spores yields was achieved in comparison to single use. Temperatures and CO2 profiles corroborates the fungus adapted differently to substrate at each usage. Such results are valuable for industrial producers of commercial formulations of the fungus spores, allowing process modernization by using packed-bed bioreactors and production costs and rice demand reduction by recycling the substrate.

中文翻译：

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以水稻为基质连续栽培填充床生物反应器中绿僵菌分生孢子的生产

摘要 本文介绍了绿僵菌 IBCB 425 孢子生产规模扩大和成本降低的研究，绿僵菌是用于甘蔗作物的昆虫病原真菌。水稻与甘蔗渣 (9:1 w/w) 混合作为基质组成，确保足够的物理结构用于填充床栽培。在台式填充床生物反应器中，仅稻米的孢子产量是从 9:1 w/w 混合物中获得的孢子产量的 56%。与生物工业中使用的塑料包装相比，在由圆柱形夹套模块构建的台式和中试规模的生物反应器中，每克底物的孢子产量相等，可以更好地控制操作和环境变量，重复之间的差异很小。尽管在中试规模的生物反应器内发生了不可忽视的温升（比理想值高 5°C），但与实验室规模相比，孢子生产并未受到损害。通过重复使用水稻最多连续种植 3 次，与单次使用相比，孢子产量增加了 2.5 倍。温度和 CO2 分布证实了真菌在每次使用时对底物的不同适应。这些结果对于真菌孢子商业配方的工业生产商很有价值，允许通过使用填充床生物反应器实现工艺现代化，并通过回收基质减少生产成本和大米需求。温度和 CO2 分布证实了真菌在每次使用时对底物的不同适应。这些结果对于真菌孢子商业配方的工业生产商很有价值，允许通过使用填充床生物反应器实现工艺现代化，并通过回收基质减少生产成本和大米需求。温度和 CO2 分布证实了真菌在每次使用时对底物的不同适应。这些结果对于真菌孢子商业配方的工业生产商很有价值，允许通过使用填充床生物反应器实现工艺现代化，并通过回收基质减少生产成本和大米需求。