Porcine parvovirus (PPV) is one of the major causes of reproductive pig disease. Due to its serious nature, wide spread and consequent great damage to the swine industry, an effective, rapid and convenient method for its detection is needed. A loop-mediated isothermal amplification (LAMP) assay was established to detect PPV infection. Two pairs of primers were specifically designed to recognize the six different sequences of open reading frame1 (ORF1) gene. The optimized LAMP program was as follows: 50 min at 59 °C followed by 3 min at 80 °C.The amplified products were analyzed both by visual inspection after staining with SYBR Green I dye and by conventional agarose gel electrophoresis. Both methods showed the same sensitivity. The limit of detection (LOD) for PPV by LAMP was 10 copies, which is 100-fold lower than conventional PCR. Our LAMP assay did not cross-react with other viruses. We used the established LAMP system to test 1100 field samples and detected 660 positives. The LAMP detection method for PPV represents a visual, sensitive and rapid assay which can detect the virus in the field, offering an attractive alternative for the PPV detection methods currently in use.

猪细小病毒（PPV）是生殖猪疾病的主要原因之一。由于其严重的性质，广泛的传播以及随之而来的对养猪业的巨大破坏，需要一种有效，快速，方便的检测方法。建立了环介导的等温扩增（LAMP）测定法来检测PPV感染。专门设计了两对引物以识别开放阅读框架1（ORF1）基因的六个不同序列。优化的LAMP程序如下：在59°C下50分钟，然后在80°C下3分钟。通过SYBR Green I染料染色后的目视检查和常规琼脂糖凝胶电泳对扩增产物进行分析。两种方法均显示出相同的灵敏度。LAMP对PPV的检出限（LOD）为10个拷贝，比常规PCR低100倍。我们的LAMP分析未与其他病毒交叉反应。我们使用已建立的LAMP系统测试1100个现场样品并检测到660个阳性样品。用于PPV的LAMP检测方法代表了一种视觉，灵敏和快速的检测方法，可以现场检测病毒，为当前使用的PPV检测方法提供了有吸引力的替代方法。