Binol-pyrene conjugate (BPC), as fluorescent probe was synthesized by multi-step synthetic strategies. BPC acts as a single sensor for dual analyte detection by a turn “on-off” mechanism for Ag⁺ and CO₃²⁻ ions. In the emission spectra, the monomer peaks of BPC were drastically quenched by the addition of Ag⁺ and CO₃²⁻ ions, due to the reverse photo-induced electron transfer (PET) process, excited-state deactivation via vibration and rotation of binaphthyl ring and the heavy atom effect responsible for the spin forbidden process. The probe BPC is highly selective and sensitive to Ag⁺ and CO₃²⁻ ions even in the presence of various interfering cations and anions at the physiological pH. The similar sensing strategy of BPC for Ag⁺ and CO₃²⁻ ions were further applied into the living cells, and their limit of detection (LOD) downs to 1.2 × 10^-7 M and 14 × 10^-6 M, respectively, which are close to the World Health Organization (WHO) drinking water standards. Furthermore, the binding interaction between the probe BPC with Ag⁺ and CO₃²⁻ ions and dynamic mode of quenching are evidenced by Stern-Volmer plots, quantum yields, NMR and DFT analysis.

通过多步合成策略合成了作为荧光探针的双酚-re共轭物（BPC）。BPC通过“开-关”机制对Ag ⁺和CO ₃ ^2-离子充当双重分析物检测的单个传感器。在发射光谱中，由于反向光致电子转移（PET）过程，通过振动和双萘基旋转引起的激发态失活，BPC的单体峰通过添加Ag ⁺和CO ₃ ^2-离子而彻底淬灭。环和重原子效应是自旋禁止过程的原因。探针BPC具有高度的选择性并对Ag ⁺敏感甚至在生理pH值下存在各种干扰阳离子和阴离子的情况下，CO ₃ ^2-离子也是如此。的类似感知策略BPC为银⁺和CO ₃ ^2-离子被进一步施加到活细胞，和它们的检测限（LOD）的极限起伏至1.2×10 ^-7 M和14×10 ^-6男，分别，这接近世界卫生组织（WHO）的饮用水标准。此外，Stern-Volmer图，量子产率，NMR和DFT分析证明了探针BPC与Ag ⁺和CO ₃ ^2-离子之间的结合相互作用和猝灭的动态模式。