Journal of Chromatography A ( IF 4.1 ) Pub Date : 2020-07-01 , DOI: 10.1016/j.chroma.2020.461383 Sandra Salido-Fortuna 1 , María Castro-Puyana 2 , María Luisa Marina 2
The potential of Micellar Electrokinetic Chromatography to achieve enantiomeric separations is reviewed in this article. The separation principles and the most frequently employed separation strategies to achieve chiral separations by Micellar Electrokinetic Chromatography are described. The use of chiral micellar systems alone or combined with other micellar systems or chiral selectors, as well as of mixtures of achiral micellar systems with chiral selectors is discussed together with the effect of different additives present in the separation medium. Indirect methods based on the derivatization of analytes with chiral derivatizing reagents and the use of achiral micelles are also considered. Preconcentration techniques employed to improve sensitivity and the main approaches developed to facilitate the coupling with Mass Spectrometry are included. The most recent and relevant methodologies developed by chiral Micellar Electrokinetic Chromatography and their applications in different fields are presented.
中文翻译:
手性胶束电动色谱。
本文综述了胶束电动色谱实现对映体分离的潜力。描述了通过胶束电动色谱实现手性分离的分离原理和最常用的分离策略。讨论了单独使用手性胶束体系或与其他胶束体系或手性选择剂结合使用,以及手性胶束体系与手性选择剂的混合物的使用,以及分离介质中存在的不同添加剂的影响。还考虑了基于用手性衍生试剂衍生分析物的间接方法和使用非手性胶束。包括用于提高灵敏度的预浓缩技术以及为促进与质谱联用而开发的主要方法。