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Optimization of hollow-fiber liquid phase microextraction for polychlorinated biphenyls in human breast milk.
Journal of Chromatography A ( IF 4.1 ) Pub Date : 2020-06-30 , DOI: 10.1016/j.chroma.2020.461381
M C Villegas-Álvarez 1 , B Callejón-Leblic 1 , G Rodríguez-Moro 1 , J L Gómez-Ariza 1 , A Arias-Borrego 1 , T García-Barrera 1
Affiliation  

A reliable and sensitive analytical approach has been optimized for the extraction of seven polychlorinated biphenyls (PCBs) from human breast milk. Hollow fiber liquid phase microextraction (HF-LPME) was applied for the first time for the extraction and pre-concentration of the analytes. Analytes were separated by gas chromatography with electron capture detector (GC-μECD) for the sensitive detection and mass spectrometry for the unequivocal identification. A rotable central composite design (RCCD) was performed for the multivariate optimization of the method. The best results were obtained at 40°C during 30 min and 600 rpm of stirring speed using a hollow fiber length of 5 cm and toluene as an extractant phase and salt addition was not required. The detection limits were in the range 7-14 ng L−1 for PCBs. The coefficients of determination of the calibration curves indicated good linearity (R2> 0.96) and the enrichment factors ranged from 74-143. This type of study is of great importance due to the deleterious effect that the presence of contaminants can produce in infants health related to the immature character of the defense system. Moreover, exclusive breastfeeding is recommended by neonatologists up to six months of life and as complementary food during the first two years.



中文翻译:

中空纤维液相微萃取技术对母乳中多氯联苯的优化。

一种可靠,灵敏的分析方法已经过优化,可从母乳中提取7种多氯联苯(PCB)。中空纤维液相微萃取(HF-LPME)首次用于分析物的提取和预浓缩。使用电子捕获检测器(GC-μECD)的气相色谱法分离分析物,以进行灵敏检测,质谱分析法进行明确鉴定。进行了可旋转的中央复合设计(RCCD),以对该方法进行多变量优化。使用5 cm的中空纤维长度和甲苯作为萃取剂相,在40°C下30分钟和600 rpm的搅拌速度下可获得最佳结果。检测极限在7-14 ng L -1的范围内用于PCB。校准曲线的测定系数显示出良好的线性(R 2 > 0.96),富集系数在74-143之间。由于污染物的存在会对婴儿健康产生与防御系统未成熟特征有关的有害影响,因此这类研究非常重要。此外,新生儿科医生建议婴儿长达六个月的纯母乳喂养,并在头两年作为辅助食品。

更新日期:2020-07-01
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