An in-situ dielectric measurement was implemented on garlic clove, onion, taro and Chinese yam cells undertaken slow cooling from 2.0 to -11.0°C. The double-membrane structure of vegetable cell corresponding to cell membrane and tonoplast was identified by splitting up one overlapping β-relaxation into two independent relaxations, and a double–shell model was applied to analyze the two dielectric relaxations based on interface polarization mechanism. The dielectric relaxation time (τ) of vegetable cells were found to respond sensitively to the freezing treatment. An abrupt shift of τ demonstrated the formation of extracellular ice, and the disappearance of the lower-frequency relaxation at the ice crystallization point was attributed to the frozen damage of cell integrity.

对蒜瓣，洋葱，芋头和山药细胞进行了原位介电测量，并将其从2.0缓​​慢冷却至-11.0℃。通过将一个重叠的β-弛豫分裂为两个独立的弛豫，来鉴定对应于细胞膜和液泡膜的蔬菜细胞的双膜结构，并基于界面极化机理，应用双壳模型分析了这两个介电弛豫。发现植物细胞的介电弛豫时间（τ）对冷冻处理敏感。τ的突变表明细胞外冰的形成，而冰晶点低频松弛的消失则归因于细胞完整性的冷冻损伤。