An acidic heteropolysaccharide HEP-2 was obtained from blue honeysuckle berry extracts after purification using Sepharose 6B and Sephadex G-200 column chromatography. The molecular weight of HEP-2, determined with high-performance gel permeation chromatography, was 3.01 × 10⁶ Da. Fourier transform infrared spectroscopy, gas chromatography and nuclear magnetic resonance analyses revealed the presence of α-, β-pyranose ring and six sugar residues in HEP-2. Amorphous aggregates of HEP-2 with irregular shape and lacking the triple helical structure were detected using the Congo red test, scanning electron and atomic force microscopy, and X-ray diffraction studies. Rheological characterization showed typical shear-thinning behavior and viscoelastic property of the polysaccharide. HEP-2 exerted significant inhibitory effects on α-amylase and α-glucosidase and displayed competitive inhibition kinetics. The collective results support the potential utility of HEP-2 as a hypoglycemic agent for enzyme-targeted treatment of diabetes or functional food.

使用Sepharose 6B和Sephadex G-200柱色谱法纯化后，从蓝色金银花浆果提取物中获得酸性杂多糖HEP-2。用高效凝胶渗透色谱法测定的HEP-2分子量为3.01×10 ⁶  Da。傅里叶变换红外光谱，气相色谱和核磁共振分析表明存在α-，β-吡喃糖环和HEP-2中的六个糖残基。使用刚果红试验，扫描电子和原子力显微镜以及X射线衍射研究检测到了具有不规则形状且缺乏三重螺旋结构的HEP-2非晶态聚集体。流变学特性表明该多糖具有典型的剪切稀化行为和粘弹性。HEP-2对α-淀粉酶和α-葡萄糖苷酶具有显着的抑制作用，并显示出竞争抑制动力学。总体结果支持HEP-2作为降糖药用于酶靶向治疗糖尿病或功能性食品的潜在用途。