The fungal kingdom has been widely studied as a source of bioactive compounds of interest to the pharmaceutical and food industry. This paper studies the production of natural red pigments by Fusarium solani BRM054066 in the submerged fermentation system, using Doehlert experimental design to determine optimal cultivation conditions. The chemical composition of the red pigment was determined by Nuclear Magnetic Resonance spectroscopy (NMR) and Ultra-Performance Liquid Chromatography coupled to Mass Spectrometry (UPLC-MS). Antioxidant activity was assessed by the ability to sequester of free radical DPPH. In the analysis of anti-inflammatory activity, murine peritoneal macrophages activated by LPS were used, and the gene expression of TNF-α, IL-1β, IL-6, IL-10 and IL-17 was determined using qPCR. As a result, it was found that agitation at 200 rpm and glucose concentration ≥ 20 g/L promote the best results in the production of red pigment. The chemical compounds identified were two naphthoquinones, fusarubin and dihydrofusarubin, and an anthraquinone, a bostrycoidin, being fusarubin the majority compound. The red pigment showed antioxidant activity by scavenge 50% of the DPPH radical, in a concentration of 24 µg/mL. The pigment also showed an effective anti-inflammatory capacity by reducing the overexpression of the pro-inflammatory cytokines TNF-α, IL-1β and IL-6 and promoting the production of anti-inflammatory IL-10 and IL-17, in murine macrophages activated by LPS (p < 0.05). According to the results, the fungus F. solani BRM054066, under optimized conditions of cultivation, proved to be a promising source of biologically active natural pigments with wide industrial applicability.

真菌界已被广泛研究为制药和食品工业感兴趣的生物活性化合物的来源。本文研究了茄镰刀菌（Fusarium solani）生产天然红色颜料的方法BRM054066在深层发酵系统中，采用多勒特实验设计确定最佳培养条件。红色颜料的化学组成通过核磁共振波谱（NMR）和超高效液相色谱-质谱联用（UPLC-MS）确定。通过螯合自由基DPPH的能力来评估抗氧化活性。在抗炎活性分析中，使用了由LPS激活的小鼠腹膜巨噬细胞，并使用qPCR测定了TNF-α，IL-1β，IL-6，IL-10和IL-17的基因表达。结果发现，在200 rpm搅拌和葡萄糖浓度≥20 g / L时，红色颜料的生产效果最好。鉴定出的化合物为两种萘醌，富沙红和二氢富沙红，以及蒽醌，Bostrycoidin，是氟沙林的主要成分。红色颜料以24 µg / mL的浓度清除了50％的DPPH自由基，从而显示出抗氧化活性。该色素还通过减少鼠巨噬细胞中促炎细胞因子TNF-α，IL-1β和IL-6的过表达并促进抗炎IL-10和IL-17的产生而显示出有效的抗炎能力。由LPS激活（p <0.05）。根据结果​​，真菌 在被LPS激活的小鼠巨噬细胞中，IL-1β和IL-6以及促进抗炎性IL-10和IL-17的产生（p <0.05）。根据结果​​，真菌 在被LPS激活的小鼠巨噬细胞中，IL-1β和IL-6以及促进抗炎性IL-10和IL-17的产生（p <0.05）。根据结果​​，真菌在优化的培养条件下，茄镰孢BRM054066被证明是具有广泛工业适用性的生物活性天然色素的有前途的来源。