The electrochemical DNA biosensor has been developed for the detection of Listeria monocytogenes in raw milk samples. The electrochemical studies of the developed biosensor was recorded by cyclic voltammetry (CV) and electrochemical impedance (EI) using methylene blue (MB) and potassium ferricyanide K₃Fe(CN)⁻₆ as redox indicators. The selectivity of the developed biosensor was demonstrated using complementary and mismatch oligonucleotide sequences. The sensitivity (S) of the developed sensor was recorded as 3461 (μA/cm²)/ng and limit of detection (LOD) was found to be 82 fg/6 µl with the regression coefficient (R²) 0.941 using CV. The sensor was characterized by field emission scanning electron microscopy (FE-SEM). The electrode was found to be stable for six months, with only 10% loss in the initial CV current.

电化学DNA生物传感器已被开发用于检测生乳样品中的单核细胞增生李斯特菌。发达生物传感器的电化学研究通过循环伏安法（CV）和电化学阻抗（EI）使用亚甲基蓝（MB）和铁氰化钾ķ记录₃的Fe（CN）^- ₆作为氧化还原指示剂。使用互补和错配的寡核苷酸序列证明了开发的生物传感器的选择性。所开发传感器的灵敏度（S）记录为3461（μA/ cm ²）/ ng，检测极限（LOD）为82 fg / 6 µl，回归系数为（R ²），使用CV为0.941。该传感器通过场发射扫描电子显微镜（FE-SEM）进行了表征。发现电极可以稳定六个月，初始CV电流仅损失10％。