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Comparative transcriptome analysis of the rice leaf folder (Cnaphalocrocis medinalis) to heat acclimation.
BMC Genomics ( IF 4.4 ) Pub Date : 2020-06-30 , DOI: 10.1186/s12864-020-06867-6
Peng-Qi Quan 1 , Ming-Zhu Li 1 , Gao-Rong Wang 1 , Ling-Ling Gu 1 , Xiang-Dong Liu 1
Affiliation  

The rice leaf folder Cnaphalocrocis medinalis Güenée is a serious insect pest of rice in Asia. This pest occurs in summer, and it is sensitive to high temperature. However, the larvae exhibit heat acclimation/adaptation. To understand the underlying mechanisms, we established a heat-acclimated strain via multigenerational selection at 39 °C. After heat shock at 41 °C for 1 h, the transcriptomes of the heat-acclimated (S-39) and unacclimated (S-27) larvae were sequenced, using the unacclimated larvae without exposure to 41 °C as the control. Five generations of selection at 39 °C led larvae to acclimate to this heat stress. Exposure to 41 °C induced 1160 differentially expressed genes (DEGs) between the heat-acclimated and unacclimated larvae. Both the heat-acclimated and unacclimated larvae responded to heat stress via upregulating genes related to sensory organ development and structural constituent of eye lens, whereas the unacclimated larvae also upregulated genes related to structural constituent of cuticle. Compared to unacclimated larvae, heat-acclimated larvae downregulated oxidoreductase activity-related genes when encountering heat shock. Both the acclimated and unacclimated larvae adjusted the longevity regulating, protein processing in endoplasmic reticulum, antigen processing and presentation, MAPK and estrogen signaling pathway to responsed to heat stress. Additionally, the unacclimated larvae also adjusted the spliceosome pathway, whereas the heat-acclimated larvae adjusted the biosynthesis of unsaturated fatty acids pathway when encountering heat stress. Although the heat-acclimated and unacclimated larvae upregulated expression of heat shock protein genes under heat stress including HSP70, HSP27 and CRYAB, their biosynthesis, metabolism and detoxification-related genes expressed differentially. The rice leaf folder larvae could acclimate to a high temperature via multigenerational heat selection. The heat-acclimated larvae induced more DEGs to response to heat shock than the unacclimated larvae. The changes in transcript level of genes were related to heat acclimation of larvae, especially these genes in sensory organ development, structural constituent of eye lens, and oxidoreductase activity. The DEGs between heat-acclimated and unacclimated larvae after heat shock were enriched in the biosynthesis and metabolism pathways. These results are helpful to understand the molecular mechanism underlying heat acclimation of insects.

中文翻译:

水稻叶夹(Cnaphalocrocis medinalis)进行热适应的转录组比较分析。

稻纵卷叶C Cnaphalocrocis medinalisGüenée是亚洲水稻的一种严重害虫。这种害虫发生在夏天,并且对高温敏感。然而,幼虫表现出热适应性/适应性。为了了解潜在的机制,我们通过在39°C的多代选择建立了一个热适应的菌株。在41°C下热激1 h后,使用未暴露于41°C的未驯化幼虫对热适应(S-39)和未适应(S-27)幼虫的转录组进行测序。在39°C下进行了五代选择,使幼虫适应了这种热应激。暴露于41°C会诱导热适应和未适应幼虫之间的1160个差异表达基因(DEG)。热适应和未适应的幼虫均通过上调与感觉器官发育和眼透镜的结构组成有关的基因来响应热应激,而未适应的幼虫也上调与表皮的结构组成有关的基因。与未适应的幼虫相比,受热适应的幼虫在遇到热激时会下调与氧化还原酶活性相关的基因。适应和不适应的幼虫都可以调节寿命调节,内质网中的蛋白质加工,抗原加工和呈递,MAPK和雌激素信号通路来响应热应激。另外,未驯化的幼虫也调节了剪接体途径,而热适应的幼虫在遇到热应激时也调节了不饱和脂肪酸途径的生物合成。尽管热适应和未适应的幼虫在热胁迫下上调了热休克蛋白基因的表达,包括HSP70,HSP27和CRYAB,但它们的生物合成,代谢和排毒相关基因却差异表达。通过多代热选择,稻叶折叠幼虫可以适应高温。热适应的幼虫比未适应的幼虫诱导更多的DEGs对热激的反应。基因转录水平的变化与幼虫的热适应有关,特别是这些基因在感觉器官发育,眼晶状体结构和氧化还原酶活性方面。热激后热适应和未适应的幼虫之间的DEG丰富了生物合成和代谢途径。
更新日期:2020-06-30
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