In this study, chitosan nanoparticles containing atorvastatin calcium were prepared using the nanospray-drying method to be used in the local treatment of peripheral nerve injuries. The main focus was to investigate the effect of the molecular weight and concentration of the polymer, the concentration of the active pharmaceutical ingredient and the diameter of the spray nozzle (4·0, 5·5 and 7·0 μm) on the final properties of nanoparticles. Atorvastatin nanoparticles were characterized in terms of morphology, particle size, polydispersity index (PDI), zeta potential, encapsulation efficiency and in vitro release. In all formulations, the nanoparticles were found to be in the submicrometer range (510·5 ± 22·3–820·0 ± 98·7 nm) with a positive surface charge (11·1–26·6 mV) and a narrow particle size distribution (PDI = 0·10–0·58). Further evaluation of the synthesized nanoparticles revealed high encapsulation efficiency (23·37–53·61%) and production yield (58·30–74·10%). Critical examination of the morphology of nanoparticles in all formulations indicated that the nanoparticles were almost spherical in shape and had a wrinkled surface. The in vitro release test revealed that the nanoparticles were capable of maintaining a sustained release of atorvastatin for about 200 h. The cytotoxicity of chitosan nanoparticles was evaluated using L-929 and B35 cells, and the nanoparticles were found to show no toxic effect.

中文翻译：

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阿托伐他汀负载纳米喷雾壳聚糖纳米粒治疗周围神经损伤

在这项研究中，使用纳米喷雾干燥法制备了含有阿托伐他汀钙的壳聚糖纳米颗粒，用于局部治疗周围神经损伤。主要焦点是研究聚合物的分子量和浓度，活性药物成分的浓度以及喷嘴直径（4·0、5·5和7·0μm）对最终性能的影响。纳米粒子。阿托伐他汀纳米颗粒的形态，粒径，多分散指数（PDI），ζ电势，包封效率和体外释放均得到了表征。在所有配方中，发现纳米颗粒都在亚微米范围内（510·5±22·3–820·0±98·7 nm），具有正表面电荷（11·1-26·6 mV）且窄粒径分布（PDI = 0·10-0·58）。对合成纳米粒子的进一步评估显示出高的包封效率（23·37–53·61％）和生产产率（58·30–74·10％）。对所有制剂中纳米颗粒的形态进行的严格检查表明，纳米颗粒的形状几乎为球形，并且表面起皱。体外释放试验表明，纳米颗粒能够维持阿托伐他汀持续释放约200小时。使用L-929和B35细胞评估了壳聚糖纳米颗粒的细胞毒性，发现该纳米颗粒没有毒性作用。对所有制剂中纳米颗粒形态的严格检查表明，纳米颗粒的形状几乎是球形的，并且表面起皱。体外释放试验表明，纳米颗粒能够维持阿托伐他汀持续释放约200小时。使用L-929和B35细胞评估了壳聚糖纳米颗粒的细胞毒性，发现该纳米颗粒没有毒性作用。对所有制剂中纳米颗粒形态的严格检查表明，纳米颗粒的形状几乎是球形的，并且表面起皱。体外释放试验表明，纳米颗粒能够维持阿托伐他汀持续释放约200小时。使用L-929和B35细胞评估了壳聚糖纳米颗粒的细胞毒性，发现该纳米颗粒没有毒性作用。