Benzodiazepines are important prescription pharmaceuticals used to help in the treatment of anxiety and sleep disorders. However, they also have a strong potential for abuse. In this respect, illicit benzodiazepines, i.e. not prescribed in Australia and designer benzodiazepines, which are new compounds that are not legally prescribed in any jurisdiction, have emerged in the illicit Australian market in recent years. Designer benzodiazepines are a new class of new psychoactive substances (NPS) and are particularly dangerous due to limited toxicity information and propensity to be mistaken for conventional benzodiazepines, leading to severe side effects and potentially death. It is therefore important to assess the prevalence of the use of these compounds in the community. The current work presents a validated liquid chromatography-mass spectrometry method for 20 prescribed and designer benzodiazepines and metabolites: 7-amino nimetazepam, alpha-hydroxy alprazolam, alprazolam, clonazepam, delorazepam, deschloroetizolam, diazepam, diclazepam, etizolam, flubromazepam, flunitrazepam, lorazepam, lormetazepam, meclonazepam, midazolam, nimetazepam, nitrazepam, oxazepam, pyrazolam and temazepam. Quetiapine, a prescription sedative drug that has been diverted for non-medical use, was also validated. Limits of quantification were predominantly below 10 ng L⁻¹, except for the ubiquitous oxazepam, quetiapine and temazepam, which were between 75–300 ng L⁻¹. Stability, recovery and matrix effects were also examined. Finally, this method was applied to influent wastewater from South Australia, which showed the presence of many benzodiazepines including the NPS etizolam.

中文翻译：

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进水废水中规定和设计的苯并二氮杂和代谢物的测定。

苯二氮卓类药物是重要的处方药，可用于治疗焦虑症和睡眠障碍。但是，它们也很容易被滥用。在这方面，非法苯二氮卓类，即近年来，在澳大利亚非法市场上出现了未在澳大利亚开出处方的苯并二氮杂designer和设计师二苯并二氮杂designer。设计师苯二氮卓类药物是一类新型的新型精神活性物质（NPS），由于毒性信息有限以及被误认为是常规苯二氮卓类药物的倾向，因此特别危险，这会导致严重的副作用甚至可能导致死亡。因此，重要的是评估社区中这些化合物的使用情况。当前的工作提出了一种验证的液相色谱-质谱法，用于20种处方药和设计者苯并二氮杂s和代谢物：7-氨基尼美西epa，α-羟基阿普唑仑，阿普唑仑，氯硝西am，地洛西p，去氯乙唑仑，地西epa，地克西p，依替莫仑，氟溴西epa 氟硝西epa，劳拉西m，洛美他西,、氯硝西p，咪达唑仑，尼美他西,、硝西epa，奥沙西m，吡唑仑和替马西m。喹硫平是一种处方镇静药物，已被转用于非医疗用途，也得到了验证。定量限主要在10 ng L以下^-1，除了普遍存在的奥沙西m，喹硫平和替马西m介于75-300 ng L ^-1之间。还检查了稳定性，回收率和基质效应。最后，该方法应用于南澳大利亚州的进水废水，该废水表明存在许多苯二氮卓类，包括NPS乙唑仑。