Adenosine plays an important role in the physiological and pathological conditions of the body by combining different types of adenosine receptors widely distributed in various tissues in the body. In present study, an acute model for paraquat-poisoning in Caenorhabditis elegans was established for quantitative assessment via a time-dose-mortality (TDM) modeling technique with various paraquat doses over 8 hours. Adenosine was first used to precondition at high, medium, and low concentrations and the survival rate of C. elegans was recorded to evaluate adenosine antistress protection against paraquat damage. The results revealed that the TDM model was good for the quantitative assessment of paraquat-poisoning on C. elegans based on the Hosmer-Lemeshow test for homogeneity of modeling (P = .38). The survival rates of adenosine-preconditioned C. elegans have a dose-dependent association with adenosine concentration. At 3000 μM (high concentration) and 300 μM (medium concentration), adenosine-preconditioned C. elegans still had survival rates of 5.38% ± 1.68% and 5.0% ± 1.19% in the subsequent 8 hours observation period. On the contrary, the survival rates of those receiving 30 μM (low concentration) and the 0 μM (unpreconditioned treatment) were zero. To conclude, adenosine preconditioning had protective effects on C. elegans intoxicated with paraquat by decreasing its mortality rate.

腺苷通过结合广泛分布在人体各个组织中的不同类型的腺苷受体，在人体的生理和病理状况中起重要作用。在本研究中，建立了秀丽隐杆线虫中百草枯中毒的急性模型，用于通过时间剂量死亡率（TDM）建模技术对百草枯剂量进行8小时以上的定量评估。腺苷首先用于高，中，低浓度的预处理，记录线虫的存活率以评估腺苷对百草枯的抗应激保护作用。结果表明，TDM模型对秀丽隐杆线虫百草枯中毒的定量评估有好处。基于Hosmer-Lemeshow检验的建模均匀性（P = .38）。腺苷预处理的秀丽隐杆线虫的存活率与腺苷浓度呈剂量依赖性。在3000μM（高浓度）和300μM（中等浓度）下，腺苷预处理的秀丽隐杆线虫在随后的8小时观察期内仍具有5.38％±1.68％和5.0％±1.19％的存活率。相反，接受30μM（低浓度）和0μM（未经预处理）的患者的存活率为零。总之，腺苷预处理通过降低百草枯的死亡率对秀丽隐杆线虫具有保护作用。