In this report, we describe the development and testing of a new coated plate which improves the sensitivity and accuracy in matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-ToF-MS). The coated plate was covered with a thin layer of hydrophobic silicon dioxide, which enabled sample enrichment due to the water repellent nature of the silicon dioxide surface. Sensitivity and required laser strengths were tested using peptide standards, with the results that these coated plates required lower laser power and showed increased sensitivity than that of common plates. Accuracy was tested using bacteria, saliva, and serum samples. The coated plates showed significantly increased degrees of accuracy through their capacity to reduce mass shift. The importance and necessity of accuracy analysis in the assessment of new sample plates, which is rarely described in other papers, is also discussed.

中文翻译：

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用于肽分析的新型二氧化硅包覆的MALDI-ToF样品板。

在此报告中，我们描述了一种新型涂层板的开发和测试，该涂层板可提高基质辅助激光解吸/电离飞行时间质谱（MALDI-ToF-MS）的灵敏度和准确性。涂覆的板被疏水性二氧化硅薄层覆盖，由于二氧化硅表面的疏水性质，其能够富集样品。使用肽标准品测试了灵敏度和所需的激光强度，结果表明，这些涂覆的板与普通板相比，所需的激光功率更低，并且灵敏度更高。使用细菌，唾液和血清样本测试准确性。涂层板通过减少质量偏移的能力显示出显着提高的准确性。