The development from single shot basic laser plasma interaction research toward experiments in which repetition rated laser-driven ion sources can be applied requires technological improvements. For example, in the case of radio-biological experiments, irradiation duration and reproducible controlled conditions are important for performing studies with a large number of samples. We present important technological advancements of recent years at the ATLAS 300 laser in Garching near Munich since our last radiation biology experiment. Improvements range from target positioning over proton transport and diagnostics to specimen handling. Exemplarily, we show the current capabilities by performing an application oriented experiment employing the zebrafish embryo model as a living vertebrate organism for laser-driven proton irradiation. The size, intensity, and energy of the laser-driven proton bunches resulted in evaluable partial body changes in the small (<1 mm) embryos, confirming the feasibility of the experimental system. The outcomes of this first study show both the appropriateness of the current capabilities and the required improvements of our laser-driven proton source for in vivo biological experiments, in particular the need for accurate, spatially resolved single bunch dosimetry and image guidance.

中文翻译：

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激光加速质子照射斑马鱼胚胎的可行性研究

从单次基础激光等离子体相互作用研究发展到可以应用重复额定激光驱动离子源的实验，需要技术改进。例如，在放射生物学实验的情况下，辐照持续时间和可重现的受控条件对于进行大量样品的研究很重要。自上次辐射生物学实验以来，我们在慕尼黑附近加兴的 ATLAS 300 激光器上展示了近年来的重要技术进步。改进范围从质子传输和诊断上的目标定位到样本处理。例如，我们通过使用斑马鱼胚胎模型作为激光驱动质子辐照的活脊椎动物生物体进行面向应用的实验来展示当前的能力。尺寸，激光驱动质子束的强度和能量导致小（<1 毫米）胚胎中可评估的部分身体变化，证实了实验系统的可行性。第一项研究的结果显示了当前能力的适当性和我们的激光驱动质子源在体内生物实验中所需的改进，特别是需要准确的、空间分辨的单束剂量测定和图像引导。