After nearly 15 years since its initial debut, super-resolution localization microscopy that surpasses the diffraction-limited resolution barrier of optical microscopy has rapidly gotten out of the ivory tower and entered a new phase to address various challenging biomedical questions. Recent advances in this technology greatly increased the imaging throughput, improved the imaging quality, simplified the sample preparation, and reduced the system cost, making this technology suitable for routine biomedical research. We will provide our perspective on the recent technical advances and their implications in serving the community of biomedical research.

中文翻译：

---

超分辨率定位显微镜：迈向高通量、高质量和低成本

自首次亮相以来近15年后，超越光学显微镜衍射极限分辨率障碍的超分辨率定位显微镜已迅速走出象牙塔，进入解决各种具有挑战性的生物医学问题的新阶段。该技术的最新进展极大地提高了成像通量、提高了成像质量、简化了样品制备并降低了系统成本，使得该技术适用于常规生物医学研究。我们将就最新的技术进步及其对服务生物医学研究界的影响提供我们的观点。