当前位置: X-MOL 学术Phycologia › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Microsatellite design for species delimitation and insights into ploidy for the Lake Baikal Cladophoraceae species flock
Phycologia ( IF 1.6 ) Pub Date : 2020-06-30 , DOI: 10.1080/00318884.2020.1771524
Sergio Díaz Martínez 1, 2 , Christian Boedeker 1 , Giuseppe C. Zuccarello 1
Affiliation  

ABSTRACT Ancient lakes are centres of adaptive radiation and speciation. The Cladophoraceae endemic to ancient Lake Baikal is a morphologically diverse group nested within Rhizoclonium that may represent a case of sympatric speciation. Recent research using ribosomal DNA markers indicates that these taxa form a monophyletic group but was not able to resolve boundaries between all of the investigated morphospecies due to very low genetic diversity. For this reason, a population genetics approach using more variable markers was investigated. In this study, we developed a set of microsatellites (SSRs) using high-throughput sequencing (HTS) data obtained from three morphospecies of Cladophoraceae from Lake Baikal. To increase amplification rate of the microsatellites across taxa, we performed an in silico cross-validation step comparing the microsatellites retrieved from three HTS datasets and tested the most promising loci on 14 of the mostly endemic morphospecies. We obtained 11 SSRs that cross-amplified among morphospecies, eight SSRs in 12 taxa and three in only four taxa. Our results showed that most loci had more than two alleles, but also displayed variation between and within morphospecies. These results indicate that this group may have gone through polyploidisation. Polyploid systems require a different approach from standard population genetic analyses. We produced ‘allelic phenotypes’ (presence/absence matrices) to analyse genetic diversity. We showed that similarity indices mostly grouped morphospecies, suggesting that this scoring method will be useful in species delimitation, but further work is needed to elucidate the speciation process in this algal species flock in Lake Baikal.

中文翻译:

用于物种定界的微卫星设计和对贝加尔湖枝藻科物种群的倍性的洞察

摘要 古代湖泊是适应性辐射和物种形成的中心。古代贝加尔湖特有的枝藻科是一个形态多样的群落,嵌套在根瘤菌内,可能代表了同域物种形成的一个例子。最近使用核糖体 DNA 标记的研究表明,这些分类群形成了一个单系群,但由于遗传多样性非常低,无法解决所有研究的形态种之间的界限。为此,研究了使用更多可变标记的群体遗传学方法。在这项研究中,我们使用从贝加尔湖枝藻科的三个形态种获得的高通量测序 (HTS) 数据开发了一组微卫星 (SSR)。为了提高微卫星跨分类群的扩增率,我们进行了计算机交叉验证步骤,比较了从三个 HTS 数据集中检索到的微卫星,并测试了 14 个主要地方性形态种中最有希望的位点。我们获得了 11 个在形态种间交叉扩增的 SSR,12 个分类群中的 8 个 SSR 和仅四个分类群中的 3 个。我们的结果表明,大多数基因座具有两个以上的等位基因,但也显示出形态种之间和内部的变异。这些结果表明该组可能经历了多倍化。多倍体系统需要不同于标准群体遗传分析的方法。我们制作了“等位基因表型”(存在/不存在矩阵)来分析遗传多样性。我们表明相似性指数主要对形态种进行分组,这表明这种评分方法将在物种定界中有用,
更新日期:2020-06-30
down
wechat
bug