Abstract

Three compounds with diuretic potential were identified from the 95% ethanol extract of Pyrrosia petiolosa (Christ) Ching. Among them, one was a new benzanilide named petiolide A (1), and the other two were phenolic derivatives barbatic acid (2) and kaempferol (3). Their structures were elucidated based on extensive spectral analyses and comparison with the literature data. The docking experiments of all compounds into the active site of the With-No-Lysine kinase 1 (WNK1) domain demonstrated that kaempferol (3) was the most effective component with diuretic potential for its comparative diuretic effect to that of an orally bioavailable WNK inhibitor WNK463 (docking score −10.99 vs −11.09).

摘要

从Pyrrosia petiolosa (Christ) Ching的 95% 乙醇提取物中鉴定出三种具有利尿潜力的化合物。其中，一种是新的苯甲酰苯胺，命名为petiolide A ( 1 )，另外两种是酚类衍生物巴巴二酸( 2 )和山奈酚( 3 )。基于广泛的光谱分析和与文献数据的比较，阐明了它们的结构。所有化合物与无赖氨酸激酶 1 (WNK1) 结构域活性位点的对接实验表明，山奈酚 ( 3 ) 是最有效的具有利尿潜力的成分，因为其利尿作用与口服生物可利用的 WNK 抑制剂相比。 WNK463（对接得分 -10.99 对 -11.09）。